The peroxin Pex3p initiates membrane assembly in peroxisome biogenesis

Kamran Ghaedi, Shigehiko Tamura, Kanji Okumoto, Yuji Matsuzono, Yukio Fujiki

研究成果: ジャーナルへの寄稿記事

86 引用 (Scopus)

抄録

Rat cDNA encoding a 372-amino-acid peroxin was isolated, primarily by functional complementation screening, using a peroxisome-deficient Chinese hamster ovary cell mutant, ZPG208, of complementation group 17. The deduced primary sequence showed ~25% amino acid identity with the yeast Pex3p, thereby we termed this cDNA rat PEX3 (RnPEX3). Human and Chinese hamster Pex3p showed 96 and 94% identity to rat Pex3p and had 373 amino acids. Pex3p was characterized as an integral membrane protein of peroxisomes, exposing its N- and C-terminal parts to the cytosol. A homozygous, inactivating missense mutation, G to A at position413, in a codon (GGA) for Gly138 and resulting in a codon (GAA) for Glu was the genetic cause of peroxisome deficiency of complementation group 17 ZPG208. The peroxisome-restoring activity apparently required the full length of Pex3p, whereas its N-terminal part from residues 1 to 40 was sufficient to target a fusion protein to peroxisomes. We also demonstrated that Pex3p binds the farnesylated peroxisomal membrane protein Pex19p. Moreover, upon expression of PEX3 in ZPG208, peroxisomal membrane vesicles were assembled before the import of soluble proteins such as PTS2-tagged green fluorescent protein. Thus, Pex3p assembles membrane vesicles before the matrix proteins are translocated.

元の言語英語
ページ(範囲)2085-2102
ページ数18
ジャーナルMolecular biology of the cell
11
発行部数6
DOI
出版物ステータス出版済み - 6 2000

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Peroxisomes
Membranes
Cricetulus
Amino Acids
Codon
Membrane Proteins
Complementary DNA
Proteins
Missense Mutation
Green Fluorescent Proteins
Cytosol
Ovary
Yeasts

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

これを引用

The peroxin Pex3p initiates membrane assembly in peroxisome biogenesis. / Ghaedi, Kamran; Tamura, Shigehiko; Okumoto, Kanji; Matsuzono, Yuji; Fujiki, Yukio.

:: Molecular biology of the cell, 巻 11, 番号 6, 06.2000, p. 2085-2102.

研究成果: ジャーナルへの寄稿記事

Ghaedi, Kamran ; Tamura, Shigehiko ; Okumoto, Kanji ; Matsuzono, Yuji ; Fujiki, Yukio. / The peroxin Pex3p initiates membrane assembly in peroxisome biogenesis. :: Molecular biology of the cell. 2000 ; 巻 11, 番号 6. pp. 2085-2102.
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abstract = "Rat cDNA encoding a 372-amino-acid peroxin was isolated, primarily by functional complementation screening, using a peroxisome-deficient Chinese hamster ovary cell mutant, ZPG208, of complementation group 17. The deduced primary sequence showed ~25{\%} amino acid identity with the yeast Pex3p, thereby we termed this cDNA rat PEX3 (RnPEX3). Human and Chinese hamster Pex3p showed 96 and 94{\%} identity to rat Pex3p and had 373 amino acids. Pex3p was characterized as an integral membrane protein of peroxisomes, exposing its N- and C-terminal parts to the cytosol. A homozygous, inactivating missense mutation, G to A at position413, in a codon (GGA) for Gly138 and resulting in a codon (GAA) for Glu was the genetic cause of peroxisome deficiency of complementation group 17 ZPG208. The peroxisome-restoring activity apparently required the full length of Pex3p, whereas its N-terminal part from residues 1 to 40 was sufficient to target a fusion protein to peroxisomes. We also demonstrated that Pex3p binds the farnesylated peroxisomal membrane protein Pex19p. Moreover, upon expression of PEX3 in ZPG208, peroxisomal membrane vesicles were assembled before the import of soluble proteins such as PTS2-tagged green fluorescent protein. Thus, Pex3p assembles membrane vesicles before the matrix proteins are translocated.",
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