The possible role of EWS-Fli1 in evasion of senescence in Ewing family tumors

Tomoya Matsunobu, Kazuhiro Tanaka, Tomoyuki Nakamura, Fumihiko Nakatani, Riku Sakimura, Masuo Hanada, Xu Li, Takamitsu Okada, Yoshinao Oda, Masazumi Tsuneyoshi, Yukihide Iwamoto

研究成果: Contribution to journalArticle査読

42 被引用数 (Scopus)

抄録

The chromosomal translocation t(11;22) yields the EWS-Fli1 fusion gene and is associated with oncogenesis of Ewing family tumors (EFT). In this study, using the RNA interference method, we show that EWS-Fli1-targeting small interfering RNAs (siRNA) depleted EWS-Fli1 protein and caused growth inhibition in EFT cells with the accumulation of p27 protein and the down-regulation of Skp2 protein in dose-dependent, time-dependent, and sequence-specific manners. Depletion of EWS-Fli1 subacutely elicited a senescence-like phenotype, but not apoptosis, in EFT cells. Furthermore, not only the knockdown of p27, but also the forced expression of Skp2, reduced the expression levels of p27 protein and partially rescued senescence-like phenotype caused by EWS-Fli1-targeting siRNAs. The accumulation of p27 protein in EWS-Fli1-depleted cells inhibited cdk2 kinase activity and was related to the stability of p27 protein, which resulted from a decrease in Skp2 protein. Immunohistochemical analysis of p27 and Skp2 proteins in EFT samples revealed that there was an inverse relationship between the expression profiles of p27 and Skp2 proteins. These findings indicate an important role of EWS-Fli1 in the prevention of senescence, leading to the unlimited growth and oncogenesis of EFT cells through a decrease in the stability of p27 protein due to increased action of Skp2-mediated 26S proteasome degradation.

本文言語英語
ページ(範囲)803-811
ページ数9
ジャーナルCancer Research
66
2
DOI
出版ステータス出版済み - 1 15 2006

All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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