The postsynaptic density and dendritic raft localization of PSD-Zip70, which contains an N-myristoylation sequence and leucine-zipper motifs

Daijiro Konno, Ji Ae Ko, Shinichi Usui, Kei Hori, Hisato Maruoka, Makoto Inui, Takashi Fujikado, Yasuo Tano, Tatsuo Suzuki, Koujiro Tohyama, Kenji Sobue

研究成果: Contribution to journalReview article査読

24 被引用数 (Scopus)

抄録

The postsynaptic site of the excitatory synapse, which is composed of the postsynaptic density (PSD) attached to the postsynaptic membrane, is a center for synaptic plasticity. To reveal the molecular organization and functional regulation of the postsynaptic site, we cloned a 70 kDa protein that is concentrated in PSDs using a monoclonal antibody against the PSD. This protein, named PSD-Zip70, is highly homologous to the human FEZ1/LZTS1 gene product. PSD-Zip70 contains an N-myristoylation consensus sequence, a polybasic cluster in the N-terminal region and four leucine-zipper motifs in the C-terminal region. Light and electron microscopy showed that this protein was localized to the dendritic spines, especially in the PSD and the postsynaptic membrane. Fractionation of the synaptic plasma membrane demonstrated that PSD-Zip70 was localized to the PSD and the dendritic raft. In Madin-Darby canine kidney (NMCK) cells, exogenous PSD-Zip70 was targeted to the apical plasma membrane of microvilli, and its N-myristoylation was necessary for this targeting. In hippocampal neurons, N-myristoylation was also required for the membrane localization and the C-terminal region was critically involved in the synaptic targeting. These results suggest that PSD-Zip70 may be involved in the dynamic properties of the structure and function of the postsynaptic site.

本文言語英語
ページ(範囲)4695-4706
ページ数12
ジャーナルJournal of cell science
115
23
DOI
出版ステータス出版済み - 12 1 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 細胞生物学

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