TY - JOUR
T1 - The prevalence of foodborne pathogenic bacteria on cutting boards and their ecological correlation with background biota
AU - Abdul-Mutalib, Noor Azira
AU - Nordin, Syafinaz Amin
AU - Osman, Malina
AU - Roslan, Ahmad Muhaimin
AU - Ishida, Natsumi
AU - Sakai, Kenji
AU - Tashiro, Yukihiro
AU - Tashiro, Kosuke
AU - Maeda, Toshinari
AU - Shirai, Yoshihito
N1 - Funding Information:
We would like to thank all researchers and students at Kyushu University, Japan that had helped us in the analysis of data. Our gratitude also goes to Dr. Mohd Huzairi Mohd Zainudin and Environmental Biotechnology Group, UPM (Prof. Dr Mohd Ali Hassan) for advising and guiding us throughout this study.
Publisher Copyright:
© 2016 Yoshihito Shirai, et al., licensee AIMS Press.
PY - 2016
Y1 - 2016
N2 - This study implemented the pyrosequencing technique and real-time quantitative PCR to determine the prevalence of foodborne pathogenic bacteria (FPB) and as well as the ecological correlations of background biota and FPB present on restaurant cutting boards (CBs) collected in Seri Kembangan, Malaysia. The prevalence of FPB in high background biota (HBB) was lower (0.24%) compared to that of low background biota (LBB) (0.54%). In addition, a multiple linear regression analysis indicated that only HBB had a significant ecological correlation with FPB. Furthermore, statistical analysis revealed that the combinations of Clostridiales, Flavobacteriales, and Lactobacillales orders in HBB had significant negative associations with FPB, suggesting that these bacteria may interact to ensure survivability and impair the growth of pathogenic bacteria.
AB - This study implemented the pyrosequencing technique and real-time quantitative PCR to determine the prevalence of foodborne pathogenic bacteria (FPB) and as well as the ecological correlations of background biota and FPB present on restaurant cutting boards (CBs) collected in Seri Kembangan, Malaysia. The prevalence of FPB in high background biota (HBB) was lower (0.24%) compared to that of low background biota (LBB) (0.54%). In addition, a multiple linear regression analysis indicated that only HBB had a significant ecological correlation with FPB. Furthermore, statistical analysis revealed that the combinations of Clostridiales, Flavobacteriales, and Lactobacillales orders in HBB had significant negative associations with FPB, suggesting that these bacteria may interact to ensure survivability and impair the growth of pathogenic bacteria.
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U2 - 10.3934/MICROBIOL.2016.2.138
DO - 10.3934/MICROBIOL.2016.2.138
M3 - Article
AN - SCOPUS:85084982235
VL - 2
SP - 138
EP - 151
JO - AIMS Microbiology
JF - AIMS Microbiology
SN - 2471-1888
IS - 2
ER -