The quantity and duration of FcRγ signals determine mast cell degranulation and survival

Sho Yamasaki, Eri Ishikawa, Masayuki Kohno, Takashi Saito

    研究成果: Contribution to journalArticle査読

    56 被引用数 (Scopus)

    抄録

    Immunoglobulin E (IgE) bound to multivalent antigen (Ag) elicits mast cell degranulation but not survival; on the contrary, IgE in the absence of Ag (IgE(-Ag)) induces survival only but not degranulation. Although these distinct responses are mediated through the same receptor, FcεRI, the molecular mechanism generating the divergence is largely unknown. We recently showed that the signals through FcRγ chain are essential for IgE(-Ag)-induced mast cell survival as well as IgE(+Ag)-induced degranulation. To determine whether the cellular output is regulated by the quantity of FcRγ signal, we expressed CD8/FcRγ chimeras (CD8/γ) in bone marrow-derived mast cells (BMMCs) from FcRγ-/- mice to manipulate the strength of FcRγ signals by anti-CD8 cross-linking. Cross-linking of CD8/γ induced mast cell survival and degranulation. Survival was induced by weaker stimulation than needed for degranulation in terms of anti-CD8 concentration and the valency of chimera. However, sustained extracellular signal-regulated kinase (Erk) activation seems to regulate survival even when the activation signal was strong enough to elicit degranulation. Generation of sustained Erk activation by active mitogen-activated protein kinase kinase (MEK) induced BMMC survival. These results suggest that the duration and the magnitude of FcRγ signals may determine mast cell survival and degranulation, respectively.

    本文言語英語
    ページ(範囲)3093-3101
    ページ数9
    ジャーナルBlood
    103
    8
    DOI
    出版ステータス出版済み - 4 15 2004

    All Science Journal Classification (ASJC) codes

    • 生化学
    • 免疫学
    • 血液学
    • 細胞生物学

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