We have studied DNA strand exchange reactions (SERs) between double stranded (ds) DNA and single-stranded (ss) DNA. Recently, we have demonstrated that cationic comb-type copolymers (CCCs) accelerated the SER by more than four orders. It is widely known that DNA binding ligands can change the structures and the properties of DNAs in a sequence-dependent or -independent manner. Then we have studied the influence of these ligands on the kinetics of the DNA strand exchange reaction. Though either Distamycin or Hoechst 33258 showed a minor effect on the DNA melting, these ligands significantly affected on the kinetics of the SER. This might suggest that we could analyze DNA binding ligands using the SER.
|出版ステータス||出版済み - 12 1 2006|
|イベント||55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, 日本|
継続期間: 9 20 2006 → 9 22 2006
|その他||55th Society of Polymer Science Japan Symposium on Macromolecules|
|Period||9/20/06 → 9/22/06|
All Science Journal Classification (ASJC) codes