The relationship between tissue oxygenation and redox status using magnetic resonance imaging

Fuminori Hyodo, Ryan M. Davis, Emi Hyodo, Shingo Matsumoto, Murali C. Krishna, James B. Mitchell

    研究成果: Contribution to journalArticle査読

    10 被引用数 (Scopus)

    抄録

    The recent development of a bi-modality magnetic resonance imaging/electron paramagnetic resonance imaging (MRI/EPRI) platform has enabled longitudinal monitoring of both tumor oxygenation and redox status in murine cancer models. The current study used this imaging platform to test the hypothesis that a more reducing tumor microenvironment accompanies the development of tumor hypoxia. To test this, the redox status of the tumor was measured using Tempol as a redox-sensitive MRI contrast agent, and tumor hypoxia was measured with Oxo63, which is an oxygen-sensitive EPRI spin probe. Images were acquired every 1-2 days in mice bearing SCCVII tumors. The median pO2 decreased from 14 mmHg at 7 days after tumor implantation to 7 mmHg at 15 days after implantation. Additionally, the hypoxic fraction, defined as the percentage of the tumor that exhibited a pO2<10 mmHg, increased with tumor size (from 10% at 500 mm3 to 60% at 3,500 mm3). The rate of Tempol reduction increased as a function of tumor volume (0.4 min-1 at 500 mm 3 to 1.7 min-1 at 3,500 mm3), suggesting that the tumor microenvironment became more reduced as the tumor grew. The results show that rapid Tempol reduction correlates with decreased tumor oxygenation, and that the Tempol decay rate constant may be a surrogate marker for tumor hypoxia.

    本文言語英語
    ページ(範囲)2103-2108
    ページ数6
    ジャーナルInternational journal of oncology
    41
    6
    DOI
    出版ステータス出版済み - 12 2012

    All Science Journal Classification (ASJC) codes

    • Oncology
    • Cancer Research

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