The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction

Hiromasa Shikata, Mami Shibata, Tomokazu Ushijima, Moeko Nakashima, Sam Geun Kong, Ken Matsuoka, Chentao Lin, Tomonao Matsushita

研究成果: Contribution to journalArticle査読

33 被引用数 (Scopus)

抄録

Plants monitor the light environment through informational photoreceptors that include phytochromes. In seedling de-etiolation, phytochrome B (phyB), which is the most important member of the phytochrome family, interacts with transcription factors to regulate gene expression and transduce light signals. In this study, we identified rrc1 (reduced red-light responses in cry1cry2 background 1), an Arabidopsis mutant that is impaired in phyB-mediated light responses. A genetic analysis demonstrated that RRC1 affected light signaling in a phyB-dependent manner. RRC1 encodes an ortholog of the human potential splicing factor SR140. The RRC1 polypeptide contains a C-terminal arginine/serine-rich (RS) domain that is important for the regulation of alternative splicing. Although the complete loss of RRC1 caused pleiotropic developmental abnormalities, the deletion of the RS domain specifically reduced phyB signaling and caused aberrant alternative splicing of several SR protein genes. Moreover, semi-quantitative RT-PCR analysis revealed that the alternative splicing patterns of some of the SR protein genes were altered in a red-light-dependent manner, and that these responses were reduced in both phyB and rrc1 mutants. These findings suggest that the regulation of alternative splicing by the RS domain of RRC1 plays an important role in phyB signal transduction.

本文言語英語
ページ(範囲)727-738
ページ数12
ジャーナルPlant Journal
70
5
DOI
出版ステータス出版済み - 6 2012

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

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