In a previous study, we isolated a Schizosaccharomyces pombe mutant defective in protein galactosylation. From an S. pombe genomic library, we cloned the gms1+ gene which restored the galactosylation of cell wall glycoproteins. Gms1 protein shares significant sequence similarity with human UDP-galactose and murine CMP-sialic acid transporters. The fission yeast strains deleted for the gms1+ gene lacked galactose residues in sell surface glycoproteins and were significantly decreased in UDP-galactose transport activity. These results showed that the gms1+ encodes an UDP-galactose transporter, and this protein appears to be an essential role for the incorporation of UDP-galactose into the lumen of Golgi in S. pombe.
|ジャーナル||Biochemical and Biophysical Research Communications|
|出版ステータス||出版済み - 3月 6 1997|
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