TY - JOUR
T1 - The self-assembled monolayer of saccharide via click chemistry
T2 - Formation and protein recognition
AU - Miura, Yoshiko
AU - Yamauchi, Takahiro
AU - Sato, Hajime
AU - Fukuda, Tomohiro
N1 - Funding Information:
This work was supported by the Industrial Technology Research Grant Program in '03 from New Energy and Industrial Technology Development Organization (NEDO) of Japan, Mitani Foundation and Shinsedai Foundation. The authors are grateful to Prof. Yoshihiro Nishida for saccharide syntheses.
PY - 2008/3/3
Y1 - 2008/3/3
N2 - We prepared the saccharide-immobilized substrate via click chemistry. The azide-terminated saccharides were reacted by a facile metathesis reaction. The density of saccharides was controlled by the incubation time of SAM preparation. The saccharide-protein interaction was analyzed using the saccharide substrate. The interaction of the saccharide substrate with protein was strong and specific due to the glyco-cluster effects. The interaction with amyloid β was analyzed by the monosaccharide-immobilized substrate, and the sulfonated saccharides showed strong interaction.
AB - We prepared the saccharide-immobilized substrate via click chemistry. The azide-terminated saccharides were reacted by a facile metathesis reaction. The density of saccharides was controlled by the incubation time of SAM preparation. The saccharide-protein interaction was analyzed using the saccharide substrate. The interaction of the saccharide substrate with protein was strong and specific due to the glyco-cluster effects. The interaction with amyloid β was analyzed by the monosaccharide-immobilized substrate, and the sulfonated saccharides showed strong interaction.
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U2 - 10.1016/j.tsf.2007.04.061
DO - 10.1016/j.tsf.2007.04.061
M3 - Article
AN - SCOPUS:39449085568
SN - 0040-6090
VL - 516
SP - 2443
EP - 2449
JO - Thin Solid Films
JF - Thin Solid Films
IS - 9
ER -