The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate

Takahisa Anada, Takumi Arisawa, Yoshihisa Ozaki, Tohru Takarada, Yoshiki Katayama, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.

元の言語英語
ページ(範囲)2267-2273
ページ数7
ジャーナルElectrophoresis
23
発行部数14
DOI
出版物ステータス出版済み - 7 1 2002

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Capillary electrophoresis
Oligodeoxyribonucleotides
Capillary Electrophoresis
Chain length
Oncogenes
Magnesium
Assays
Genes
Ions
Ligands
Mutation
polyacrylamide

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

これを引用

The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate. / Anada, Takahisa; Arisawa, Takumi; Ozaki, Yoshihisa; Takarada, Tohru; Katayama, Yoshiki; Maeda, Mizuo.

:: Electrophoresis, 巻 23, 番号 14, 01.07.2002, p. 2267-2273.

研究成果: ジャーナルへの寄稿記事

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