The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate

Takahisa Anada, Takumi Arisawa, Yoshihisa Ozaki, Tohru Takarada, Yoshiki Katayama, Mizuo Maeda

研究成果: Contribution to journalArticle査読

28 被引用数 (Scopus)

抄録

We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.

本文言語英語
ページ(範囲)2267-2273
ページ数7
ジャーナルELECTROPHORESIS
23
14
DOI
出版ステータス出版済み - 7 2002

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

フィンガープリント 「The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル