We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.
|出版ステータス||出版済み - 7 2002|
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Clinical Biochemistry