The significance of membrane fluidity of feeder cell-derived substrates for maintenance of iPS cell stemness

Yue Zhou, Hongli Mao, Binata Joddar, Nobuhisa Umeki, Yasushi Sako, Ken Ichi Wada, Chieko Nishioka, Eiki Takahashi, Yi Wang, Yoshihiro Ito

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

The biological activity of cell-derived substrates to maintain undifferentiated murine-induced pluripotent stem (iPS) cells was correlated to membrane fluidity as a new parameter of cell culture substrates. Murine embryonic fibroblasts (MEFs) were employed as feeder cells and their membrane fluidity was tuned by chemical fixation using formaldehyde (FA). Membrane fluidity was evaluated by real-time single-molecule observations of green fluorescent protein-labeled epidermal growth factor receptors on chemically fixed MEFs. Biological activity was monitored by colony formation of iPS cells. Treatment with a low concentration of FA sustained the membrane fluidity and biological activity, which were comparable to those of mitomycin C-treated MEFs. The biological activity was further confirmed by sustained expression of alkaline phosphatase, SSEA-1, and other pluripotency markers in iPS cells after 3-5 days of culture on FA-fixed MEFs. Chemical fixation of feeder cells has several advantages such as providing ready-to-use culture substrates without contamination by proliferating feeder cells. Therefore, our results provide an important basis for the development of chemically fixed culture substrates for pluripotent stem cell culture as an alternative to conventional treatment by mitomycin C or x-ray irradiation.

本文言語英語
論文番号11386
ジャーナルScientific reports
5
DOI
出版ステータス出版済み - 6 12 2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 一般

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