The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles

Aya Yoshimura, Naoki Adachi, Hitomi Matsuno, Masaki Kawamata, Yusuke Yoshioka, Hisae Kikuchi, Haruki Odaka, Tadahiro Numakawa, Hiroshi Kunugi, Takahiro Ochiya, Yoshitaka Tamai

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Extracellular vesicles (EVs) can modulate microenvironments by transferring biomolecules, including RNAs and proteins derived from releasing cells, to target cells. To understand the molecular mechanisms maintaining the neural stem cell (NSC) niche through EVs, a new transgenic (Tg) rat strain that can release human CD63-GFP-expressing EVs from the NSCs was established. Human CD63-GFP expression was controlled under the rat Sox2 promoter (Sox2/ human CD63-GFP), and it was expressed in undifferentiated fetal brains. GFP signals were specifically observed in in vitro cultured NSCs obtained from embryonic brains of the Tg rats. We also demonstrated that embryonic NSC (eNSC)-derived EVs were labelled by human CD63-GFP. Furthermore, when we examined the transfer of EVs, eNSC-derived EVs were found to be incorporated into astrocytes and eNSCs, thus implying an EV-mediated communication between different cell types around NSCs. This new Sox2/human CD63-GFP Tg rat strain should provide resources to analyse the cell-to-cell communication via EVs in NSC microenvironments.

元の言語英語
記事番号dmm028779
ジャーナルDMM Disease Models and Mechanisms
11
発行部数1
DOI
出版物ステータス出版済み - 1 2018
外部発表Yes

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Transgenic Rats
Neural Stem Cells
Stem cells
Rats
Brain
Stem Cell Niche
Communication
Biomolecules
RNA
Extracellular Vesicles
Embryonic Stem Cells
Cell Communication
Astrocytes
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience (miscellaneous)
  • Medicine (miscellaneous)
  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles. / Yoshimura, Aya; Adachi, Naoki; Matsuno, Hitomi; Kawamata, Masaki; Yoshioka, Yusuke; Kikuchi, Hisae; Odaka, Haruki; Numakawa, Tadahiro; Kunugi, Hiroshi; Ochiya, Takahiro; Tamai, Yoshitaka.

:: DMM Disease Models and Mechanisms, 巻 11, 番号 1, dmm028779, 01.2018.

研究成果: ジャーナルへの寄稿記事

Yoshimura, A, Adachi, N, Matsuno, H, Kawamata, M, Yoshioka, Y, Kikuchi, H, Odaka, H, Numakawa, T, Kunugi, H, Ochiya, T & Tamai, Y 2018, 'The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles', DMM Disease Models and Mechanisms, 巻. 11, 番号 1, dmm028779. https://doi.org/10.1242/dmm.028779
Yoshimura, Aya ; Adachi, Naoki ; Matsuno, Hitomi ; Kawamata, Masaki ; Yoshioka, Yusuke ; Kikuchi, Hisae ; Odaka, Haruki ; Numakawa, Tadahiro ; Kunugi, Hiroshi ; Ochiya, Takahiro ; Tamai, Yoshitaka. / The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles. :: DMM Disease Models and Mechanisms. 2018 ; 巻 11, 番号 1.
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