抄録
During the process of transformation Haemophilusinfluenzae cells bind its own DNA but little or no foreign DNA. This specificity for recognition of DNA was studied by cloning Haemophilus DNA in E. coli. Haemophilus DNA fragments were cloned using plasmid pBR322 as a vector. The fragment cH7 cloned in pBR322 was found to be homologous to Haemophilus DNA and shown to bind irreversibly to competent Haemophilus cells. The fact that cH7 isolated from E. coli lacks Haemophilus modification leads to the conclusion that modification does not play a role in the uptake mechanism. Uptake specificity is a function of recognition sequences that reside in DNA itself.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 208-214 |
| ページ数 | 7 |
| ジャーナル | Biochemical and Biophysical Research Communications |
| 巻 | 88 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | 出版済み - 5 14 1979 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
これを引用
The specific uptake of cloned Haemophilus DNA. / Chung, Bon chu; Goodgal, Sol H.
：: Biochemical and Biophysical Research Communications, 巻 88, 番号 1, 14.05.1979, p. 208-214.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - The specific uptake of cloned Haemophilus DNA
AU - Chung, Bon chu
AU - Goodgal, Sol H.
PY - 1979/5/14
Y1 - 1979/5/14
N2 - During the process of transformation Haemophilusinfluenzae cells bind its own DNA but little or no foreign DNA. This specificity for recognition of DNA was studied by cloning Haemophilus DNA in E. coli. Haemophilus DNA fragments were cloned using plasmid pBR322 as a vector. The fragment cH7 cloned in pBR322 was found to be homologous to Haemophilus DNA and shown to bind irreversibly to competent Haemophilus cells. The fact that cH7 isolated from E. coli lacks Haemophilus modification leads to the conclusion that modification does not play a role in the uptake mechanism. Uptake specificity is a function of recognition sequences that reside in DNA itself.
AB - During the process of transformation Haemophilusinfluenzae cells bind its own DNA but little or no foreign DNA. This specificity for recognition of DNA was studied by cloning Haemophilus DNA in E. coli. Haemophilus DNA fragments were cloned using plasmid pBR322 as a vector. The fragment cH7 cloned in pBR322 was found to be homologous to Haemophilus DNA and shown to bind irreversibly to competent Haemophilus cells. The fact that cH7 isolated from E. coli lacks Haemophilus modification leads to the conclusion that modification does not play a role in the uptake mechanism. Uptake specificity is a function of recognition sequences that reside in DNA itself.
UR - http://www.scopus.com/inward/record.url?scp=0018366339&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018366339&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(79)91717-0
DO - 10.1016/0006-291X(79)91717-0
M3 - Article
C2 - 378229
AN - SCOPUS:0018366339
VL - 88
SP - 208
EP - 214
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -