The suppressive effect of peritoneal exudate macrophages on production of antibody to sheep erythrocytes in vitro

Yasunobu Yoshikai, Shunji Miake, Masatoshi Sano, Kikuo Nomoto

    研究成果: ジャーナルへの寄稿記事

    11 引用 (Scopus)

    抄録

    The effects of peritoneal exudate macrophages on antibody response to sheep erythrocytes (SRBC) were investigated in mice. Peritoneal exudate macrophages obtained from mice injected intraperitoneally with proteose peptone or Corynebacterium parvum 4 days earlier had stronger ability to phagocytize and degrade SRBC than normal resident macrophages. These macrophages suppressed antibody formation to SRBC in vitro as well as in vivo. This suppression was overridden by increasing the amount of SRBC and diminished completely by pretreatment of the macrophages with iodoacetate and partly by pretreatment with 2-deoxyglucose, both known to be inhibitors of phagocytosis, but not by addition of indomethacin to the in vitro culture. These results suggest that the suppression of antibody response by peritoneal exudate macrophages was due to the increased activity of these cells as scavenger cells, resulting in a reduced amount of effective antigenic stimulation, and that it was not mediated by a prostaglandin-dependent mechanism. The scavenger function of these macrophages may be due to Ia-negative macrophages.

    元の言語英語
    ページ(範囲)266-278
    ページ数13
    ジャーナルCellular Immunology
    77
    発行部数2
    DOI
    出版物ステータス出版済み - 4 15 1983

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    Peritoneal Macrophages
    Exudates and Transudates
    Antibody Formation
    Sheep
    Erythrocytes
    Macrophages
    Iodoacetates
    Propionibacterium acnes
    Deoxyglucose
    Phagocytosis
    Indomethacin
    Prostaglandins
    In Vitro Techniques

    All Science Journal Classification (ASJC) codes

    • Immunology

    これを引用

    The suppressive effect of peritoneal exudate macrophages on production of antibody to sheep erythrocytes in vitro. / Yoshikai, Yasunobu; Miake, Shunji; Sano, Masatoshi; Nomoto, Kikuo.

    :: Cellular Immunology, 巻 77, 番号 2, 15.04.1983, p. 266-278.

    研究成果: ジャーナルへの寄稿記事

    Yoshikai, Yasunobu ; Miake, Shunji ; Sano, Masatoshi ; Nomoto, Kikuo. / The suppressive effect of peritoneal exudate macrophages on production of antibody to sheep erythrocytes in vitro. :: Cellular Immunology. 1983 ; 巻 77, 番号 2. pp. 266-278.
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    abstract = "The effects of peritoneal exudate macrophages on antibody response to sheep erythrocytes (SRBC) were investigated in mice. Peritoneal exudate macrophages obtained from mice injected intraperitoneally with proteose peptone or Corynebacterium parvum 4 days earlier had stronger ability to phagocytize and degrade SRBC than normal resident macrophages. These macrophages suppressed antibody formation to SRBC in vitro as well as in vivo. This suppression was overridden by increasing the amount of SRBC and diminished completely by pretreatment of the macrophages with iodoacetate and partly by pretreatment with 2-deoxyglucose, both known to be inhibitors of phagocytosis, but not by addition of indomethacin to the in vitro culture. These results suggest that the suppression of antibody response by peritoneal exudate macrophages was due to the increased activity of these cells as scavenger cells, resulting in a reduced amount of effective antigenic stimulation, and that it was not mediated by a prostaglandin-dependent mechanism. The scavenger function of these macrophages may be due to Ia-negative macrophages.",
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