The thermostability of DNA-binding protein HU from bacilli

Keith S. Wilson, Constantin E. Vorgias, Isao Tanaka, Stephen W. White, Makoto Kimura

研究成果: ジャーナルへの寄稿記事

40 引用 (Scopus)

抄録

The primary and tertiary structures of DNA-binding protein HU from Bacillus stearothermophilus are already known. The primary structure has been previously determined for HU from the closely related B.globigii and the determinations of the sequences from B.caldolyticus and B.subtilis are described here. These bacteria have optimum growth temperatures of > 70°C (B.caldolyticus), 65°C (B.stearothermophilus), 37°C (B.subtilis) and 30°C (B.globigii). in vitro measurements from circular dichroic spectra described here give Tmvalues reflecting these growth temperatures, of 68, 64, 43 and 41°C respectively. We discuss here the relative thermostability of the four proteins in terms of the amino acid differences between the sequences and the three-dimensional model of the B.stearothermophilus HU. The current model for the interaction of the protein with DNA is only discussed in terms of its relevance with regard to thermostability.

元の言語英語
ページ(範囲)11-22
ページ数12
ジャーナルProtein Engineering, Design and Selection
4
発行部数1
DOI
出版物ステータス出版済み - 1 1 1990

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DNA-Binding Proteins
Growth temperature
Bacilli
Bacillus
DNA
Proteins
Geobacillus stearothermophilus
Temperature
Growth
Sequence Analysis
Amino acids
Bacteria
Amino Acids
Carrier Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Molecular Biology

これを引用

Wilson, K. S., Vorgias, C. E., Tanaka, I., White, S. W., & Kimura, M. (1990). The thermostability of DNA-binding protein HU from bacilli. Protein Engineering, Design and Selection, 4(1), 11-22. https://doi.org/10.1093/protein/4.1.11

The thermostability of DNA-binding protein HU from bacilli. / Wilson, Keith S.; Vorgias, Constantin E.; Tanaka, Isao; White, Stephen W.; Kimura, Makoto.

:: Protein Engineering, Design and Selection, 巻 4, 番号 1, 01.01.1990, p. 11-22.

研究成果: ジャーナルへの寄稿記事

Wilson, KS, Vorgias, CE, Tanaka, I, White, SW & Kimura, M 1990, 'The thermostability of DNA-binding protein HU from bacilli', Protein Engineering, Design and Selection, 巻. 4, 番号 1, pp. 11-22. https://doi.org/10.1093/protein/4.1.11
Wilson, Keith S. ; Vorgias, Constantin E. ; Tanaka, Isao ; White, Stephen W. ; Kimura, Makoto. / The thermostability of DNA-binding protein HU from bacilli. :: Protein Engineering, Design and Selection. 1990 ; 巻 4, 番号 1. pp. 11-22.
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