The volume-activated chloride current in human endothelial cells depends on intracellular ATP

Masahiro Oike, Guy Droogmans, Bernd Nilius

研究成果: Contribution to journalArticle

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We have studied the effect of intracellular ATP on volume-activated Cl--currents in endothelial cells from human umbilical veins by means of the whole-cell patch clamp technique. The run-down of this current in ruptured patches during repetitive applications of hypotonic solutions (HTS) could be significantly reduced if the cells were internally perfused with a pipette solution that contained 4 mmol/l ATP. This run-down was much less pronounced if currents were recorded using nystatin-perforated patches. The amplitude of the current was drastically reduced and its activation became slower if the cells were superfused with a glucose-free medium with 1 mmol/l KCN. Adding 4 mmol/l ATPγS, a poorly hydrolyzable ATP-analogue, to the patch pipette prevented run-down of the current during repetitive activations by HTS, even if the cells were superfused with glucose-free solution with 1 mmol/l KCN. It is concluded that activation of the mechanosensitive Cl- conductance in human endothelial cells requires the presence of intracellular ATP, but not its hydrolysis.

元の言語英語
ページ(範囲)184-186
ページ数3
ジャーナルPflügers Archiv European Journal of Physiology
427
発行部数1-2
DOI
出版物ステータス出版済み - 5 1 1994
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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