The WD40 protein Morg1 facilitates Par6-aPKC binding to Crb3 for apical identity in epithelial cells

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

Formation of apico-basal polarity in epithelial cells is crucial for both morphogenesis (e.g., cyst formation) and function (e.g., tight junction development). Atypical protein kinase C (aPKC), complexed with Par6, is considered to translocate to the apical membrane and function in epithelial cell polarization. However, the mechanism for translocation of the Par6-aPKC complex has remained largely unknown. Here, we show that the WD40 protein Morg1 (mitogen-activated protein kinase organizer 1) directly binds to Par6 and thus facilitates apical targeting of Par6-aPKC in Madin-Darby canine kidney epithelial cells. Morg1 also interacts with the apical transmembrane protein Crumbs3 to promote Par6- aPKC binding to Crumbs3, which is reinforced with the apically localized small GTPase Cdc42. Depletion of Morg1 disrupted both tight junction development in monolayer culture and cyst formation in three-dimensional culture; apico-basal polarity was notably restored by forced targeting of aPKC to the apical surface. Thus, Par6-aPKC recruitment to the premature apical membrane appears to be required for definition of apical identity of epithelial cells.

元の言語英語
ページ(範囲)635-650
ページ数16
ジャーナルJournal of Cell Biology
200
発行部数5
DOI
出版物ステータス出版済み - 3 1 2013

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Epithelial Cells
Proteins
Tight Junctions
Cysts
Madin Darby Canine Kidney Cells
Membranes
Monomeric GTP-Binding Proteins
Mitogen-Activated Protein Kinases
Morphogenesis
PKC-3 protein

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

The WD40 protein Morg1 facilitates Par6-aPKC binding to Crb3 for apical identity in epithelial cells. / Hayase, Junya; Kamakura, Sachiko; Iwakiri, Yuko; Yamaguchi, Yoshihiro; Izaki, Tomoko; Ito, Takashi; Sumimoto, Hideki.

:: Journal of Cell Biology, 巻 200, 番号 5, 01.03.2013, p. 635-650.

研究成果: ジャーナルへの寄稿記事

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AU - Izaki, Tomoko

AU - Ito, Takashi

AU - Sumimoto, Hideki

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