Theaflavins inhibit glucose transport across Caco-2 cells through the downregulation of the Ca2+/AMP-activated protein kinase-mediated glucose transporter SGLT1

Baorui Li, Lei Fu, Chizumi Abe, Alexia M. Nectoux, Ayaka Yamamoto, Toshiro Matsui

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)

抄録

The study investigated the effects of theaflavins, which are intestinally non-absorbable compounds, on intestinal glucose transport in Caco-2 cells. 13C6-Glucose transport experiments clearly revealed that glucose transport across Caco-2 cells was significantly (p < 0.01) inhibited by theaflavins. When Caco-2 cells were treated with 40 μM theaflavins for 24 h, the expression of SGLT1 expression was significantly (p < 0.05) suppressed, whereas no difference in GLUT2 expression was observed. The theaflavin-induced inhibition of glucose transport was reversed by the inhibition of influx routes mediated by OATP and MCT transporters. A Wes analysis established that theaflavin-induced phosphorylation of AMPK was significantly (p < 0.05) suppressed by the inhibition of endoplasmic reticulum Ca2+-release and CaMKK β. These findings demonstrated for the first time that theaflavins can inhibit glucose transport across Caco-2 cell monolayers through the suppression of SGLT1 expression partly via the activation of the intracellular Ca2+/CaMKK β/AMPK signaling pathway.

本文言語英語
論文番号104273
ジャーナルJournal of Functional Foods
75
DOI
出版ステータス出版済み - 12月 2020

!!!All Science Journal Classification (ASJC) codes

  • 食品科学
  • 医学(その他)
  • 栄養および糖尿病

フィンガープリント

「Theaflavins inhibit glucose transport across Caco-2 cells through the downregulation of the Ca2+/AMP-activated protein kinase-mediated glucose transporter SGLT1」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル