Thermococcus coalescens sp. nov., a cell-fusing hyperthermophilic archaeon from Suiyo Seamount

Tomohiko Kuwabara, Masaomi Minaba, Yukihiro Iwayama, Isao Inouye, Miwako Nakashima, Katsumi Marumo, Akihiko Maruyama, Akihiko Sugai, Toshihiro Itoh, Jun Ichiro Ishibashi, Tetsuro Urabe, Masahiro Kamekura

研究成果: Contribution to journalArticle査読

35 被引用数 (Scopus)

抄録

A cell-fusing hyperthermophilic archaeon was isolated from hydrothermal fluid obtained from Suiyo Seamount of the Izu-Bonin Arc. The isolate, TS1T, is an irregular coccus, usually 0.5-2 μm in diameter and motile with a polar tuft of flagella. Cells in the exponential phase of growth fused at room temperature in the presence of DNA-intercalating dye to become as large as 5 μm in diameter. Fused cells showed dark spots that moved along in the cytoplasm. Large cells with a similar appearance were also observed upon culture at 87 °C, suggesting the occurrence of similar cell fusions during growth. Transmission electron microscopy revealed that cells in the exponential phase possessed a thin and electron-lucent cell envelope that could be lost subsequently during culture. The fragile cell envelope must be related to cell fusion. The cells grew at 57-90 °C, pH 5.2-8.7 and at NaCl concentrations of 1.5-4.5%, with the optima being 87 °C, pH 6.5 and 2.5% NaCl. The isolate was an anaerobic chemo-organotroph that grew on either yeast extract or tryptone as the sole growth substrate. The genomic DNA G+C content was 53.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate was closely related to Thermococcus species. However, no significant DNA-DNA hybridization was observed between genomic DNA of strain TS1T and phylogenetically related Thermococcus species. We propose that isolate TS1T represents a novel species, Thermococcus coalescens sp. nov., with the name reflecting the cell fusion activity observed in the strain. The type strain is TS1T (= JCM 12540T = DSM 16538T).

本文言語英語
論文番号63432
ページ(範囲)2507-2514
ページ数8
ジャーナルInternational Journal of Systematic and Evolutionary Microbiology
55
6
DOI
出版ステータス出版済み - 11 2005

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

フィンガープリント 「Thermococcus coalescens sp. nov., a cell-fusing hyperthermophilic archaeon from Suiyo Seamount」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル