Thermodynamic analysis of a multifunctional RNA-binding protein, PhoRpp38, in the hyperthermophilic archaeon Pyrococcus horikoshii OT3

Kosuke Oshima, Takashi Nakashima, Yoshimitsu Kakuta, Kouhei Tsumoto, Makoto Kimura

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

The protein component PhoRpp38 of Pyrococcus horikoshii ribonuclease P (RNase P) is known to be a multifunctional RNA-binding protein. Previous biochemical data indicate that it binds to two stem-loops in RNase P RNA (PhopRNA). Thermodynamic analysis revealed that PhoRpp38 and PhopRNA interact with each other with an association constant (Ka) of 1.56 × 10 7 M -1. It was further found that PhoRpp38 simultaneously binds two stem-loop structures in PhopRNA with approximately equal affinity. Crystals of PhoRpp38 in complex with the stem-loop were grown and diffracted to a resolution of 7.0Å on a synchrotron X-ray source.

本文言語英語
ページ(範囲)1252-1255
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
76
6
DOI
出版ステータス出版済み - 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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