Thermoresponsive biointerface with a elastin model peptide

Lipo-VPGVG2 was synthesized by the conjugation of elastin model peptide of (Val-Pro-Gly-Val-Gly)2 and a-hpoic acid. The peptide showed some changes of conformation and water solubility in the aqueous solution. The DSC results suggested the thermoresponsive transition with temperature. The peptide of Lipo-VPGVG2 was dissolved in aqueous solution, and the gold substrate was immersed in the LipoVPGVG2 solution in order to prepare the self-assembled monolayer (SAM). The SAM formation was confirmed by FTIR, XPS, and QCM. The cell attachment of fibroblast NIH 3T3 was observed on the Lipo-VPGVG2-SAM materials prepared by the dilute peptide solutions (20 and 80 nM). The cell detachment was observed on cooling the peptide SAM at 4°C. The Lipo-VPGVG2 showed a thermoresponsive biofunction.