Thermostable valyl-tRNA, isoleucyl-tRNA and methionyl-tRNA synthetases from an extreme thermophile Thermus thermophilus HB8: protein structure and Zn2+ binding

Daisuke Kohda, Shigeyuki Yokoyama, Tatsuo Miyazawa

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Thermostable valyl-tRNA, isoleucyl-tRNA and methionyl-tRNA synthetases have been purified from an extreme thermophile, Thermus thermophilus HB8. Valyl-tRNA and isoleucyl-tRNA synthetases are found to be monomer proteins (Mr 108000 and 129000, respectively), while methionyl-tRNA synthetase is a dimer protein (Mr 150000). These enzymes are very similar with respect to amino acid compositions and α-helix contents as estimated by circular dichroism analyses. Furthermore, two Zn2+ are tightly bound to each of these synthetases. These data suggest that valyl-tRNA and isoleucyl-tRNA synthetases consist of two domains, each corresponding to the subunit of methionyl-tRNA synthetase.

元の言語英語
ページ(範囲)20-23
ページ数4
ジャーナルFEBS Letters
174
発行部数1
DOI
出版物ステータス出版済み - 8 20 1984
外部発表Yes

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Methionine-tRNA Ligase
Thermus thermophilus
Transfer RNA
Isoleucine-tRNA Ligase
Proteins
Ligases
Circular Dichroism
Dimers
Monomers
Amino Acids
Enzymes
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

Thermostable valyl-tRNA, isoleucyl-tRNA and methionyl-tRNA synthetases from an extreme thermophile Thermus thermophilus HB8 : protein structure and Zn2+ binding. / Kohda, Daisuke; Yokoyama, Shigeyuki; Miyazawa, Tatsuo.

:: FEBS Letters, 巻 174, 番号 1, 20.08.1984, p. 20-23.

研究成果: ジャーナルへの寄稿記事

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abstract = "Thermostable valyl-tRNA, isoleucyl-tRNA and methionyl-tRNA synthetases have been purified from an extreme thermophile, Thermus thermophilus HB8. Valyl-tRNA and isoleucyl-tRNA synthetases are found to be monomer proteins (Mr 108000 and 129000, respectively), while methionyl-tRNA synthetase is a dimer protein (Mr 150000). These enzymes are very similar with respect to amino acid compositions and α-helix contents as estimated by circular dichroism analyses. Furthermore, two Zn2+ are tightly bound to each of these synthetases. These data suggest that valyl-tRNA and isoleucyl-tRNA synthetases consist of two domains, each corresponding to the subunit of methionyl-tRNA synthetase.",
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AU - Kohda, Daisuke

AU - Yokoyama, Shigeyuki

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PY - 1984/8/20

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