Thiol-organosilica particles internally functionalized with propidium iodide as a multicolor fluorescence and x-ray computed tomography probe and application for non-invasive functional gastrointestinal tract imaging

Michihiro Nakamura, Aziz Awaad, Koichiro Hayashi, Kazuhiko Ochiai, Kazunori Ishimura

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

Propidium iodide (PI) is a fluorescent nucleic acid dye that contains iodide molecules. Thiol-organosilica particles that were internally functionalized with PI (PI particles) were prepared for X-ray computed tomography (CT) and multicolor fluorescence imaging. PI particles of various sizes were synthesized in a one-pot process. The particles showed unique fluorescent signals and X-ray absorption, with enhancement of the fluorescence intensity of PI located inside organosilica particles. PI particles had multicolor fluorescence, including original fluorescence and near-infrared (NIR). Orally administered PI particles were observed in the gastrointestinal tract (GIT) using fluorescent imaging devices and X-ray CT. An in vivo fluorescence imaging system could detect the NIR fluorescence of PI particles in the GIT specifically. Multipurpose zoom fluorescence microscopy was used to noninvasively visualize the real-time passage of particles and the movement of the GIT. The passage and distribution of particles over time in the GIT were demonstrated using X-ray CT. A correlation analysis between the fluorescent and X-ray CT data demonstrated the characteristics, limitations, and novel potential for noninvasive functional GIT dual modal imaging.

元の言語英語
ページ(範囲)3772-3779
ページ数8
ジャーナルChemistry of Materials
24
発行部数19
DOI
出版物ステータス出版済み - 10 9 2012
外部発表Yes

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Propidium
Sulfhydryl Compounds
Tomography
Fluorescence
Imaging techniques
X rays
Infrared radiation
Fluorescence microscopy
Nucleic acids
X ray absorption
Imaging systems
Iodides
Dyes
Nucleic Acids
Molecules
Coloring Agents

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

これを引用

Thiol-organosilica particles internally functionalized with propidium iodide as a multicolor fluorescence and x-ray computed tomography probe and application for non-invasive functional gastrointestinal tract imaging. / Nakamura, Michihiro; Awaad, Aziz; Hayashi, Koichiro; Ochiai, Kazuhiko; Ishimura, Kazunori.

:: Chemistry of Materials, 巻 24, 番号 19, 09.10.2012, p. 3772-3779.

研究成果: ジャーナルへの寄稿記事

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abstract = "Propidium iodide (PI) is a fluorescent nucleic acid dye that contains iodide molecules. Thiol-organosilica particles that were internally functionalized with PI (PI particles) were prepared for X-ray computed tomography (CT) and multicolor fluorescence imaging. PI particles of various sizes were synthesized in a one-pot process. The particles showed unique fluorescent signals and X-ray absorption, with enhancement of the fluorescence intensity of PI located inside organosilica particles. PI particles had multicolor fluorescence, including original fluorescence and near-infrared (NIR). Orally administered PI particles were observed in the gastrointestinal tract (GIT) using fluorescent imaging devices and X-ray CT. An in vivo fluorescence imaging system could detect the NIR fluorescence of PI particles in the GIT specifically. Multipurpose zoom fluorescence microscopy was used to noninvasively visualize the real-time passage of particles and the movement of the GIT. The passage and distribution of particles over time in the GIT were demonstrated using X-ray CT. A correlation analysis between the fluorescent and X-ray CT data demonstrated the characteristics, limitations, and novel potential for noninvasive functional GIT dual modal imaging.",
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AU - Nakamura, Michihiro

AU - Awaad, Aziz

AU - Hayashi, Koichiro

AU - Ochiai, Kazuhiko

AU - Ishimura, Kazunori

PY - 2012/10/9

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AB - Propidium iodide (PI) is a fluorescent nucleic acid dye that contains iodide molecules. Thiol-organosilica particles that were internally functionalized with PI (PI particles) were prepared for X-ray computed tomography (CT) and multicolor fluorescence imaging. PI particles of various sizes were synthesized in a one-pot process. The particles showed unique fluorescent signals and X-ray absorption, with enhancement of the fluorescence intensity of PI located inside organosilica particles. PI particles had multicolor fluorescence, including original fluorescence and near-infrared (NIR). Orally administered PI particles were observed in the gastrointestinal tract (GIT) using fluorescent imaging devices and X-ray CT. An in vivo fluorescence imaging system could detect the NIR fluorescence of PI particles in the GIT specifically. Multipurpose zoom fluorescence microscopy was used to noninvasively visualize the real-time passage of particles and the movement of the GIT. The passage and distribution of particles over time in the GIT were demonstrated using X-ray CT. A correlation analysis between the fluorescent and X-ray CT data demonstrated the characteristics, limitations, and novel potential for noninvasive functional GIT dual modal imaging.

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