Three-dimensional analysis of collagen lamellae in the anterior stroma of the human cornea visualized by second harmonic generation imaging microscopy

Naoyuki Morishige, Yuki Takagi, Tai ichiro Chikama, Atsushi Takahara, Teruo Nishida

研究成果: Contribution to journalArticle査読

46 被引用数 (Scopus)

抄録

Purpose. The structure of collagen lamellae in the anterior stroma of the human cornea is thought to be an important determinant of corneal rigidity. The three-dimensional structure of such collagen lamellae in normal human corneas was examined. Methods. The anterior portion of 27 normal human corneas was obtained from donor tissue for Descemet's stripping automated endothelial keratoplasty (DSAEK) surgery, and blocks (~3-mm square) of the central cornea were examined by second harmonic generation (SHG) imaging microscopy. Each cornea was scanned from the surface of Bowman's layer to a depth of 150 fxm, and SHG forward signals were collected. The angles of collagen lamellae immediately below to a depth of 30 fim below Bowman's layer (sutural lamellae) as well as of those at a depth of 50 or 100 fxm were measured. The density and width of sutural lamellae were also evaluated. Results. Collagen lamellae in the anterior stroma were evenly distributed and randomly oriented. The angle of sutural lamellae relative to Bowman's layer was 19.19 ± 4.34° (mean ± SD). The angles of collagen lamellae at depths of 50 or 100;xm were 8.91 ± 2.91 and 6.91 ± 2.11°, respectively. The density of sutural lamellae was 910.0 ± 480.4/mm2, and their width was 1314 ± 5.03 and 7.11 ± 3.00;xm in the region immediately beneath and 30 fxm below Bowman's layer, respectively. Conclusions. Collagen lamellae in the anterior stroma of the normal human cornea are interwoven in three dimensions and adhere densely to Bowman's layer. This structure may contribute to the rigidity and curvature of the anterior portion of the cornea.

本文言語英語
ページ(範囲)911-915
ページ数5
ジャーナルInvestigative Ophthalmology and Visual Science
52
2
DOI
出版ステータス出版済み - 2 2011

All Science Journal Classification (ASJC) codes

  • 眼科学
  • 感覚系
  • 細胞および分子神経科学

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