Three-dimensional electron microscopy of the photosystem II core complex

Kouta Mayanagi, Takashi Ishikawa, Chikashi Toyoshima, Yorinao Inoue, Katsuyoshi Nakazato

研究成果: ジャーナルへの寄稿学術誌査読

5 被引用数 (Scopus)

抄録

A three-dimensional image of the spinach photosystem II core complex composed of CP47, D1, D2, cytochrome b-559, and psbI gene product was reconstructed at 20-Å resolution from the two-dimensional crystals negatively stained with phosphotungstate. Confirming the previous proposal, the crystal had a p22121 symmetry. One PSH core complex was measured to be 80 x 80 Å in the membrane plane and 88 Å normal to it. The mass distribution was asymmetric about the lipid bilayer, consistent with predictions from the amino acid sequences. The lumenal mass consisted of three domains forming a characteristic triangular platform with another domain on top of it. Three stromal domains were smaller and linearly arranged. Due to strong stain exclusion in the hydrophobic core part of the lipid bilayer, the transmembrane region appeared to be imaged with a reversed contrast. Inverting the contrast resulted in a reasonable density distribution for that part. Thus, though the information on the transmembrane region is limited, the domain structure of the PSII core complex was revealed and allowed us to propose a model for the arrangement of subunits in the PSII core complex.

本文言語英語
ページ(範囲)211-224
ページ数14
ジャーナルJournal of structural biology
123
3
DOI
出版ステータス出版済み - 11月 1998
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学

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