Three-dimensional structure of a Bombyx mori Omega-class glutathione transferase

Kohji Yamamoto, Mamoru Suzuki, Akifumi Higashiura, Atsushi Nakagawa

研究成果: Contribution to journalArticle査読

16 被引用数 (Scopus)

抄録

Glutathione transferases (GSTs) are major phase II detoxification enzymes that play central roles in the defense against various environmental toxicants as well as oxidative stress. Here we report the crystal structure of an Omega-class glutathione transferase of Bombyx mori, bmGSTO, to gain insight into its catalytic mechanism. The structure of bmGSTO complexed with glutathione determined at a resolution of 2.5. Å reveals that it exists as a dimer and is structurally similar to Omega-class GSTs with respect to its secondary and tertiary structures. Analysis of a complex between bmGSTO and glutathione showed that bound glutathione was localized to the glutathione-binding site (G-site). Site-directed mutagenesis of bmGSTO mutants indicated that amino acid residues Leu62, Lys65, Lys77, Val78, Glu91 and Ser92 in the G-site contribute to catalytic activity.

本文言語英語
ページ(範囲)588-593
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
438
4
DOI
出版ステータス出版済み - 9 6 2013

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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