Thy-1 antigen mediates apoptosis of rat glomerular cells in vitro and in vivo

Hiroyuki Morita, Ken Ichi Isobe, Zhe Cai, Takashi Miyazaki, Yoshihiro Matsumoto, Torn Sliinzato, Yasunobu Yoshikai, Koji Kimata, Kenji Maeda

研究成果: ジャーナルへの寄稿学術誌査読

30 被引用数 (Scopus)


Injection of anti-Thy-1 antibody into a rat induces immediate glomerular cell death and subsequent development of glomerulonephritis. Whether the immediate cell death in this model is apoptotic has yet to be determined. Recent in vivo studies on thymocyte death have elucidated that the Thy-1 molecule can activate intracellular signaling for apoptosis. This observation prompted us to re-examine whether stimulation with anti-Thy-1 antibody can provoke apoptosis in the rat glomerulus. We found that anti-Thy-1 antibody could induce laddered DNA fragmentation of isolated glomeruli and mesangial cells in culture, definite biochemical evidence for random double-stranded breaks through apoptosis. Such DNA laddering was also demonstrated in the isolated glomeruli of rats that had been infused with anti-Thy-1 antibody several hours before. Furthermore, the terminal deoxynucleotidyl-transferase-mediated oligonucleotide nick end labeling technique stained a cell in the mesangium. Although apoptosis may be considered a candidate mechanism mediating resolution of hypercellularity in the anti-Thy-1 model, we propose that it is also involved in the immediate cell death in this model.

出版ステータス出版済み - 1996

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 腎臓病学
  • 生理学(医学)
  • 泌尿器学


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