Time-resolved fluorescence spectroscopy of dopamine in the single cells

M. Mabuchi, J. I. Shimada, K. Okamoto, Y. Kawakami, S. Fujita, K. Matsushige

研究成果: ジャーナルへの寄稿Conference article

8 引用 (Scopus)

抄録

Dopamine hydrochloric acid salt in aqueous solution was excited at 266 nm Al2O3:Ti laser and the sufficient fluorescence emission peaking at 330 nm was detected with a streak camera. The fluorescence decay curve was fitted by 1-exponentional functions, with the lifetime of approximately 0.80 ns. The influence of deep-UV laser excitation on cells is also discussed for the direct observation of dopamine in the living cells. In addition, it is needed to detect the dopamine fluorescence in the living cell sensitively, and separately from emission of other fluorescent species. When instrumental arrangement and time-resolved spectral analysis can make it possible to solve such problems, direct visualization of the secretion process of individual cells will be achieved by the laser-induced native fluorescence imaging microscopy, without using any additional fluorescent probes. This quantitative imaging technique will provide a useful noninvasive approach for the study of dynamic cellular changes and the understanding of the molecular mechanisms of information transporting processes.

元の言語英語
ページ(範囲)140-148
ページ数9
ジャーナルProceedings of SPIE - The International Society for Optical Engineering
4252
DOI
出版物ステータス出版済み - 1 1 2001
イベントAdvances in Flourescence Sensing Technology V - San Jose, CA, 米国
継続期間: 1 24 20011 25 2001

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Fluorescence Spectroscopy
dopamine
Fluorescence spectroscopy
Dopamine
Fluorescence
fluorescence
Cell
cells
spectroscopy
secretions
streak cameras
hydrochloric acid
Cells
Imaging
ultraviolet lasers
UV Laser
Streak cameras
Laser-induced Fluorescence
Imaging techniques
imaging techniques

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

これを引用

Time-resolved fluorescence spectroscopy of dopamine in the single cells. / Mabuchi, M.; Shimada, J. I.; Okamoto, K.; Kawakami, Y.; Fujita, S.; Matsushige, K.

:: Proceedings of SPIE - The International Society for Optical Engineering, 巻 4252, 01.01.2001, p. 140-148.

研究成果: ジャーナルへの寄稿Conference article

Mabuchi, M. ; Shimada, J. I. ; Okamoto, K. ; Kawakami, Y. ; Fujita, S. ; Matsushige, K. / Time-resolved fluorescence spectroscopy of dopamine in the single cells. :: Proceedings of SPIE - The International Society for Optical Engineering. 2001 ; 巻 4252. pp. 140-148.
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