TIMP-3 and Phosphatidylinositol 3-kinase genes were found to be related to the progression of colon cancer in a comparison of pneumoperitoneum and laparotomy in a murine model

Fumiaki Tanaka, Hideto Sonoda, Masahiro Okamoto, Koshi Mimori, Tohru Utsunomiya, Hiroshi Inoue, Taizo Hanai, Masaki Mori

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Purpose. Laparoscopic surgery has been performed on a wide variety of patients because the clinical benefits of a perioperative recovery result in a better short-time outcome than an open colectomy. However, no basic and molecular-biological examinations have yet to elucidate the mechanisms of such an improved outcome. Method. To examine the gene expression profiles of mice inoculated with colon cancer cells, the tumors, the livers and the lungs of mice were analyzed using a microarray. Results. In the laparotomy and control group, we observed a significant enhancement of tumor growth in comparison to the laparoscopic group. When analyzing the genes that became more remarkably upregulated or downregulated in the laparoscopic group in comparison to those in the laparotomy or control group, we focused our attention on the TIMP-3 gene and PI3-kinase gene expression. The downregulation of TIMP-3 and the upregulation of PI3-kinase were observed. To confirm the downstream signal of the PI3-kinase gene, we examined the expression of Skp-2 gene and p27 protein. We observed the expression level of the Skp-2 to be upregulated, while the expression level of p27 protein decreased. Conclusions. The surgical procedures of a laparotomy induced a downregulation of TIMP-3 and an upregulation of PI3-kinase, which together resulted in an enhancement of cancer cell growth thus leading to an increased invasion activity and cell cycle in comparison to treatment using a laparoscopic approach.

元の言語英語
ページ(範囲)220-225
ページ数6
ジャーナルSurgery Today
37
発行部数3
DOI
出版物ステータス出版済み - 3 1 2007

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Tissue Inhibitor of Metalloproteinase-3
Phosphatidylinositol 3-Kinase
Pneumoperitoneum
Phosphatidylinositol 3-Kinases
Colonic Neoplasms
Laparotomy
Down-Regulation
Genes
Up-Regulation
Activity Cycles
Gene Expression
Neoplasms
Control Groups
Colectomy
Growth
Transcriptome
Laparoscopy
Cell Cycle
Proteins
Lung

All Science Journal Classification (ASJC) codes

  • Surgery

これを引用

TIMP-3 and Phosphatidylinositol 3-kinase genes were found to be related to the progression of colon cancer in a comparison of pneumoperitoneum and laparotomy in a murine model. / Tanaka, Fumiaki; Sonoda, Hideto; Okamoto, Masahiro; Mimori, Koshi; Utsunomiya, Tohru; Inoue, Hiroshi; Hanai, Taizo; Mori, Masaki.

:: Surgery Today, 巻 37, 番号 3, 01.03.2007, p. 220-225.

研究成果: ジャーナルへの寄稿記事

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abstract = "Purpose. Laparoscopic surgery has been performed on a wide variety of patients because the clinical benefits of a perioperative recovery result in a better short-time outcome than an open colectomy. However, no basic and molecular-biological examinations have yet to elucidate the mechanisms of such an improved outcome. Method. To examine the gene expression profiles of mice inoculated with colon cancer cells, the tumors, the livers and the lungs of mice were analyzed using a microarray. Results. In the laparotomy and control group, we observed a significant enhancement of tumor growth in comparison to the laparoscopic group. When analyzing the genes that became more remarkably upregulated or downregulated in the laparoscopic group in comparison to those in the laparotomy or control group, we focused our attention on the TIMP-3 gene and PI3-kinase gene expression. The downregulation of TIMP-3 and the upregulation of PI3-kinase were observed. To confirm the downstream signal of the PI3-kinase gene, we examined the expression of Skp-2 gene and p27 protein. We observed the expression level of the Skp-2 to be upregulated, while the expression level of p27 protein decreased. Conclusions. The surgical procedures of a laparotomy induced a downregulation of TIMP-3 and an upregulation of PI3-kinase, which together resulted in an enhancement of cancer cell growth thus leading to an increased invasion activity and cell cycle in comparison to treatment using a laparoscopic approach.",
author = "Fumiaki Tanaka and Hideto Sonoda and Masahiro Okamoto and Koshi Mimori and Tohru Utsunomiya and Hiroshi Inoue and Taizo Hanai and Masaki Mori",
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AU - Sonoda, Hideto

AU - Okamoto, Masahiro

AU - Mimori, Koshi

AU - Utsunomiya, Tohru

AU - Inoue, Hiroshi

AU - Hanai, Taizo

AU - Mori, Masaki

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