Tissue binding patterns and in vitro effects of campylobacter jejuni DNA-binding protein from starved cells

Hua Piao, Motozumi Minohara, Nobutoshi Kawamura, Wei Li, Takuya Matsushita, Ryo Yamasaki, Yoshimitsu Mizunoe, Jun-Ichi Kira

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

Campylobacter jejuni (C. jejuni) is frequently associated with axonal Guillain-Barré syndrome (GBS). We reported that C. jejuni DNA-binding protein from starved cells (C-Dps) binds to and damages myelinated nerves in vivo. We studied the binding patterns of C-Dps to nervous tissues and its in vitro effects on neural cells. Immunohistochemically, C-Dps labeled the nodes of Ranvier, the outermost parts of internodal myelin and the basement membrane in the peripheral nerves, and neurons and myelin in the central nervous tissues. Its binding was blocked by sulfatide. C-Dps bound to the cell surfaces of nerve growth factor (NGF)-treated PC12 cells leading to dose-dependent LDH release, which was inhibited by either heat-denaturation of C-Dps or coincubation with an anti-C-Dps mAb. However, its binding to the surfaces of cultured NSC34 cells, S16 cells, or dorsal root ganglion cells, did not induce cytotoxicity. These findings suggest a possible involvement of C-Dps in C. jejuni-related GBS.

元の言語英語
ページ(範囲)58-66
ページ数9
ジャーナルNeurochemical Research
36
発行部数1
DOI
出版物ステータス出版済み - 1 1 2011

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Campylobacter jejuni
DNA-Binding Proteins
Tissue
Sulfoglycosphingolipids
Denaturation
Nerve Growth Factor
Cytotoxicity
Nerve Tissue
Neurons
Myelin Sheath
Ranvier's Nodes
PC12 Cells
Spinal Ganglia
Peripheral Nerves
Basement Membrane
Cultured Cells
Hot Temperature
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Biochemistry

これを引用

Tissue binding patterns and in vitro effects of campylobacter jejuni DNA-binding protein from starved cells. / Piao, Hua; Minohara, Motozumi; Kawamura, Nobutoshi; Li, Wei; Matsushita, Takuya; Yamasaki, Ryo; Mizunoe, Yoshimitsu; Kira, Jun-Ichi.

:: Neurochemical Research, 巻 36, 番号 1, 01.01.2011, p. 58-66.

研究成果: ジャーナルへの寄稿記事

Piao, Hua ; Minohara, Motozumi ; Kawamura, Nobutoshi ; Li, Wei ; Matsushita, Takuya ; Yamasaki, Ryo ; Mizunoe, Yoshimitsu ; Kira, Jun-Ichi. / Tissue binding patterns and in vitro effects of campylobacter jejuni DNA-binding protein from starved cells. :: Neurochemical Research. 2011 ; 巻 36, 番号 1. pp. 58-66.
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