Titration of the Alternative Complement Pathway Activity of Representative Cultured Fishes

Tomoki Yano, Yukihiro Hatayama, Hiroko Matsuyama, Miki Nakao

研究成果: ジャーナルへの寄稿記事

35 引用 (Scopus)

抄録

Methods for assaying the alternative complement pathway activity (ACH50) of rainbow trout Salmo gairdneri, ayu Plecoglossus altivelis, carp Cyprinus carpio, tilapia Tilapia nilotica, eel Anguilla japonica, yellowtail Seriola quinqueradiata, porgy Pagrus major and flounder Paralichthys olivaceus sera were investigated. The ACH50 values of the sera of all fish except eel could be measured by incubating each serum with rabbit red blood cells (target cells) at 20°C in the presence of 10 mM EGTA and 10 mM MgCl2. The pH values and incubation periods employed were: rainbow trout, pH 7.0, 1.5 h; ayu, pH 7.0, 1.5 h; carp, pH 7.5, 1.5 h; tilapia, pH 7.0, 1.5; yellowtail, pH 7.0, 2.5 h; porgy, pH 7.5, 2.0 h; flounder, pH 7.5, 2.0 h. The hemolytic activity of eel serum was not reduced even in the presence of 10 mM EDTA, suggesting the participation of unknown factor(s) in the hemolysis. Therefore, titration of the ACH50 value of eel serum was not carried out. ACH50 values (unit/ml, mean±SD, n = 3 to 24) of the above 7 species were as follows: rainbow trout, 456±88; ayu, 880±376; carp, 51 ±14; tilapia, 684±560; yellowtail, 1016±56; porgy, 328 ±188; flounder, 508 ±60. These values are 5 to 60 times higher than those reported for mammals, suggesting that the role of the alternative pathway is much more important in fish than in mammals.

元の言語英語
ページ(範囲)1049-1054
ページ数6
ジャーナルNippon Suisan Gakkaishi (Japanese Edition)
54
発行部数6
DOI
出版物ステータス出版済み - 1 1 1988

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titration
serum
eel
complement
Plecoglossus altivelis
porgy
rainbow
fish
Oncorhynchus mykiss
flounder
tilapia (common name)
carp
mammal
EDTA
mammals
Seriola quinqueradiata
Pagrus major
Anguilla japonica
blood
incubation

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

Titration of the Alternative Complement Pathway Activity of Representative Cultured Fishes. / Yano, Tomoki; Hatayama, Yukihiro; Matsuyama, Hiroko; Nakao, Miki.

:: Nippon Suisan Gakkaishi (Japanese Edition), 巻 54, 番号 6, 01.01.1988, p. 1049-1054.

研究成果: ジャーナルへの寄稿記事

Yano, Tomoki ; Hatayama, Yukihiro ; Matsuyama, Hiroko ; Nakao, Miki. / Titration of the Alternative Complement Pathway Activity of Representative Cultured Fishes. :: Nippon Suisan Gakkaishi (Japanese Edition). 1988 ; 巻 54, 番号 6. pp. 1049-1054.
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