Titration of the Alternative Complement Pathway Activity of Representative Cultured Fishes

Tomoki Yano, Yukihiro Hatayama, Hiroko Matsuyama, Miki Nakao

研究成果: Contribution to journalArticle査読

37 被引用数 (Scopus)

抄録

Methods for assaying the alternative complement pathway activity (ACH50) of rainbow trout Salmo gairdneri, ayu Plecoglossus altivelis, carp Cyprinus carpio, tilapia Tilapia nilotica, eel Anguilla japonica, yellowtail Seriola quinqueradiata, porgy Pagrus major and flounder Paralichthys olivaceus sera were investigated. The ACH50 values of the sera of all fish except eel could be measured by incubating each serum with rabbit red blood cells (target cells) at 20°C in the presence of 10 mM EGTA and 10 mM MgCl2. The pH values and incubation periods employed were: rainbow trout, pH 7.0, 1.5 h; ayu, pH 7.0, 1.5 h; carp, pH 7.5, 1.5 h; tilapia, pH 7.0, 1.5; yellowtail, pH 7.0, 2.5 h; porgy, pH 7.5, 2.0 h; flounder, pH 7.5, 2.0 h. The hemolytic activity of eel serum was not reduced even in the presence of 10 mM EDTA, suggesting the participation of unknown factor(s) in the hemolysis. Therefore, titration of the ACH50 value of eel serum was not carried out. ACH50 values (unit/ml, mean±SD, n = 3 to 24) of the above 7 species were as follows: rainbow trout, 456±88; ayu, 880±376; carp, 51 ±14; tilapia, 684±560; yellowtail, 1016±56; porgy, 328 ±188; flounder, 508 ±60. These values are 5 to 60 times higher than those reported for mammals, suggesting that the role of the alternative pathway is much more important in fish than in mammals.

本文言語英語
ページ(範囲)1049-1054
ページ数6
ジャーナルNippon Suisan Gakkaishi (Japanese Edition)
54
6
DOI
出版ステータス出版済み - 1 1 1988

All Science Journal Classification (ASJC) codes

  • Aquatic Science

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