Toxicological analysis of chlorhexidine in human serum using HPLC on a polymer-coated ODS column

Keiko Kudo, Noriaki Ikeda, Akiko Kiyoshima, Yukiko Hino, Naoki Nishida, Naohide Inoue

研究成果: ジャーナルへの寄稿記事

23 引用 (Scopus)

抄録

A simple and reliable high-performance liquid chromatographic (HPLC) method for analyzing chlorhexidine in human serum was developed. After the addition of an internal standard, levomepromazine, 0.2 mL serum was deproteinized with 10% perchloric acid. The acidic supernatant was neutralized with 1M potassium carbonate solution, and the insoluble salt was removed by centrifugation. An aliquot of the supernatant was applied to HPLC with UV detection (260 nm). HPLC separation was achieved on a polymer-coated ODS column equilibrated with acetonitrile/water containing 0.05 % trifluoroacetic acid, 0.05% heptafluorobutyric acid, and 0.1% triethylamine (40:60, v/v). The calibration curve was linear in the concentration range from 0.05 to 50.0 μg/mL, and the lower limit of detection was 0.05 μg/mL. The accuracy and precision of the method were evaluated at concentrations of 0.5 μg/mL and 5.0 μg/mL. The coefficients of variation ranged from 4.0 to 4.5%. The concentration of chlorhexidine in the serum of a patient who died after a suspected intravenous injection of chlorhexidine gluconate was determined.

元の言語英語
ページ(範囲)119-122
ページ数4
ジャーナルJournal of Analytical Toxicology
26
発行部数2
DOI
出版物ステータス出版済み - 1 1 2002

Fingerprint

Chlorhexidine
Toxicology
serum
Polymers
polymer
liquid
acid
Liquids
Methotrimeprazine
Serum
Trifluoroacetic acid
Trifluoroacetic Acid
Potash
Acids
Centrifugation
Acetonitrile
Intravenous Injections
Calibration
Limit of Detection
potassium

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

これを引用

Toxicological analysis of chlorhexidine in human serum using HPLC on a polymer-coated ODS column. / Kudo, Keiko; Ikeda, Noriaki; Kiyoshima, Akiko; Hino, Yukiko; Nishida, Naoki; Inoue, Naohide.

:: Journal of Analytical Toxicology, 巻 26, 番号 2, 01.01.2002, p. 119-122.

研究成果: ジャーナルへの寄稿記事

Kudo, Keiko ; Ikeda, Noriaki ; Kiyoshima, Akiko ; Hino, Yukiko ; Nishida, Naoki ; Inoue, Naohide. / Toxicological analysis of chlorhexidine in human serum using HPLC on a polymer-coated ODS column. :: Journal of Analytical Toxicology. 2002 ; 巻 26, 番号 2. pp. 119-122.
@article{6f6dd5119ed9407b94917bab2ea8befa,
title = "Toxicological analysis of chlorhexidine in human serum using HPLC on a polymer-coated ODS column",
abstract = "A simple and reliable high-performance liquid chromatographic (HPLC) method for analyzing chlorhexidine in human serum was developed. After the addition of an internal standard, levomepromazine, 0.2 mL serum was deproteinized with 10{\%} perchloric acid. The acidic supernatant was neutralized with 1M potassium carbonate solution, and the insoluble salt was removed by centrifugation. An aliquot of the supernatant was applied to HPLC with UV detection (260 nm). HPLC separation was achieved on a polymer-coated ODS column equilibrated with acetonitrile/water containing 0.05 {\%} trifluoroacetic acid, 0.05{\%} heptafluorobutyric acid, and 0.1{\%} triethylamine (40:60, v/v). The calibration curve was linear in the concentration range from 0.05 to 50.0 μg/mL, and the lower limit of detection was 0.05 μg/mL. The accuracy and precision of the method were evaluated at concentrations of 0.5 μg/mL and 5.0 μg/mL. The coefficients of variation ranged from 4.0 to 4.5{\%}. The concentration of chlorhexidine in the serum of a patient who died after a suspected intravenous injection of chlorhexidine gluconate was determined.",
author = "Keiko Kudo and Noriaki Ikeda and Akiko Kiyoshima and Yukiko Hino and Naoki Nishida and Naohide Inoue",
year = "2002",
month = "1",
day = "1",
doi = "10.1093/jat/26.2.119",
language = "English",
volume = "26",
pages = "119--122",
journal = "Journal of Analytical Toxicology",
issn = "0146-4760",
publisher = "Preston Publications",
number = "2",

}

TY - JOUR

T1 - Toxicological analysis of chlorhexidine in human serum using HPLC on a polymer-coated ODS column

AU - Kudo, Keiko

AU - Ikeda, Noriaki

AU - Kiyoshima, Akiko

AU - Hino, Yukiko

AU - Nishida, Naoki

AU - Inoue, Naohide

PY - 2002/1/1

Y1 - 2002/1/1

N2 - A simple and reliable high-performance liquid chromatographic (HPLC) method for analyzing chlorhexidine in human serum was developed. After the addition of an internal standard, levomepromazine, 0.2 mL serum was deproteinized with 10% perchloric acid. The acidic supernatant was neutralized with 1M potassium carbonate solution, and the insoluble salt was removed by centrifugation. An aliquot of the supernatant was applied to HPLC with UV detection (260 nm). HPLC separation was achieved on a polymer-coated ODS column equilibrated with acetonitrile/water containing 0.05 % trifluoroacetic acid, 0.05% heptafluorobutyric acid, and 0.1% triethylamine (40:60, v/v). The calibration curve was linear in the concentration range from 0.05 to 50.0 μg/mL, and the lower limit of detection was 0.05 μg/mL. The accuracy and precision of the method were evaluated at concentrations of 0.5 μg/mL and 5.0 μg/mL. The coefficients of variation ranged from 4.0 to 4.5%. The concentration of chlorhexidine in the serum of a patient who died after a suspected intravenous injection of chlorhexidine gluconate was determined.

AB - A simple and reliable high-performance liquid chromatographic (HPLC) method for analyzing chlorhexidine in human serum was developed. After the addition of an internal standard, levomepromazine, 0.2 mL serum was deproteinized with 10% perchloric acid. The acidic supernatant was neutralized with 1M potassium carbonate solution, and the insoluble salt was removed by centrifugation. An aliquot of the supernatant was applied to HPLC with UV detection (260 nm). HPLC separation was achieved on a polymer-coated ODS column equilibrated with acetonitrile/water containing 0.05 % trifluoroacetic acid, 0.05% heptafluorobutyric acid, and 0.1% triethylamine (40:60, v/v). The calibration curve was linear in the concentration range from 0.05 to 50.0 μg/mL, and the lower limit of detection was 0.05 μg/mL. The accuracy and precision of the method were evaluated at concentrations of 0.5 μg/mL and 5.0 μg/mL. The coefficients of variation ranged from 4.0 to 4.5%. The concentration of chlorhexidine in the serum of a patient who died after a suspected intravenous injection of chlorhexidine gluconate was determined.

UR - http://www.scopus.com/inward/record.url?scp=0036192208&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036192208&partnerID=8YFLogxK

U2 - 10.1093/jat/26.2.119

DO - 10.1093/jat/26.2.119

M3 - Article

C2 - 11916014

AN - SCOPUS:0036192208

VL - 26

SP - 119

EP - 122

JO - Journal of Analytical Toxicology

JF - Journal of Analytical Toxicology

SN - 0146-4760

IS - 2

ER -