Transcriptional Activation of β-Tropomyosin Mediated by Serum Response Factor and a Novel Barx Homologue, Barx1b, in Smooth Muscle Cells

Mako Nakamura, Wataru Nishida, Shunsuke Mori, Kunio Hiwada, Ken'ichiro Hayashi, Kenji Sobue

研究成果: ジャーナルへの寄稿記事

24 引用 (Scopus)

抄録

Tropomyosin (TM) is a regulatory protein of actomyosin system. Muscle type-specific expression of TM isoforms is generated from different genes and by alternative splicing. β-TM isoforms in chicken skeletal and smooth muscles are encoded by a single gene and transcribed from the same promoter. We previously reported a smooth muscle cell (SMC) phenotype-dependent change in β-TM expression (Kashiwada, K., Nishida, W., Hayashi, K., Ozawa, K., Yamanaka, Y., Saga, H., Yamashita, T., Tohyama, M., Shimada, S., Sato, K., and Sobue, K. (1997) J. Biol. Chem. 272, 15396-15404), and identified β-TM as an SMC-differentiation marker. Here, we characterized the transcriptional machinery of the β-TM gene in SMCs. Promoter and gel mobility shift analyses revealed an obligatory role for serum response factor and its interaction with the CArG box sequence in the SMC-specific transcription of the β-TM gene in differentiated SMCs. We further isolated a novel homologue of the Barx homeoprotein family, Barx1b, from chicken gizzard. Barx1b was exclusively localized to SMCs of the upper digestive organs and their attached arteries and to craniofacial structures. Serum response factor and Barx1b bound each other directly, coordinately transactivated the β-TM gene in differentiated SMCs and heterologous cells, and formed a ternary complex with a CArG probe. Taken together, these results suggest that SRF and Barx1b are coordinately involved in the SMC-specific transcription of the β-TM gene in the upper digestive organs and their attached arteries.

元の言語英語
ページ(範囲)18313-18320
ページ数8
ジャーナルJournal of Biological Chemistry
276
発行部数21
DOI
出版物ステータス出版済み - 1 25 2001

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Serum Response Factor
Tropomyosin
Transcriptional Activation
Smooth Muscle Myocytes
Muscle
Chemical activation
Cells
Genes
Transcription
Chickens
Protein Isoforms
Arteries
Avian Gizzard
Homeodomain Proteins
Actomyosin
Differentiation Antigens
Alternative Splicing
Electrophoretic Mobility Shift Assay
Machinery
Smooth Muscle

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Transcriptional Activation of β-Tropomyosin Mediated by Serum Response Factor and a Novel Barx Homologue, Barx1b, in Smooth Muscle Cells. / Nakamura, Mako; Nishida, Wataru; Mori, Shunsuke; Hiwada, Kunio; Hayashi, Ken'ichiro; Sobue, Kenji.

:: Journal of Biological Chemistry, 巻 276, 番号 21, 25.01.2001, p. 18313-18320.

研究成果: ジャーナルへの寄稿記事

Nakamura, Mako ; Nishida, Wataru ; Mori, Shunsuke ; Hiwada, Kunio ; Hayashi, Ken'ichiro ; Sobue, Kenji. / Transcriptional Activation of β-Tropomyosin Mediated by Serum Response Factor and a Novel Barx Homologue, Barx1b, in Smooth Muscle Cells. :: Journal of Biological Chemistry. 2001 ; 巻 276, 番号 21. pp. 18313-18320.
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abstract = "Tropomyosin (TM) is a regulatory protein of actomyosin system. Muscle type-specific expression of TM isoforms is generated from different genes and by alternative splicing. β-TM isoforms in chicken skeletal and smooth muscles are encoded by a single gene and transcribed from the same promoter. We previously reported a smooth muscle cell (SMC) phenotype-dependent change in β-TM expression (Kashiwada, K., Nishida, W., Hayashi, K., Ozawa, K., Yamanaka, Y., Saga, H., Yamashita, T., Tohyama, M., Shimada, S., Sato, K., and Sobue, K. (1997) J. Biol. Chem. 272, 15396-15404), and identified β-TM as an SMC-differentiation marker. Here, we characterized the transcriptional machinery of the β-TM gene in SMCs. Promoter and gel mobility shift analyses revealed an obligatory role for serum response factor and its interaction with the CArG box sequence in the SMC-specific transcription of the β-TM gene in differentiated SMCs. We further isolated a novel homologue of the Barx homeoprotein family, Barx1b, from chicken gizzard. Barx1b was exclusively localized to SMCs of the upper digestive organs and their attached arteries and to craniofacial structures. Serum response factor and Barx1b bound each other directly, coordinately transactivated the β-TM gene in differentiated SMCs and heterologous cells, and formed a ternary complex with a CArG probe. Taken together, these results suggest that SRF and Barx1b are coordinately involved in the SMC-specific transcription of the β-TM gene in the upper digestive organs and their attached arteries.",
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AU - Nishida, Wataru

AU - Mori, Shunsuke

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AU - Hayashi, Ken'ichiro

AU - Sobue, Kenji

PY - 2001/1/25

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AB - Tropomyosin (TM) is a regulatory protein of actomyosin system. Muscle type-specific expression of TM isoforms is generated from different genes and by alternative splicing. β-TM isoforms in chicken skeletal and smooth muscles are encoded by a single gene and transcribed from the same promoter. We previously reported a smooth muscle cell (SMC) phenotype-dependent change in β-TM expression (Kashiwada, K., Nishida, W., Hayashi, K., Ozawa, K., Yamanaka, Y., Saga, H., Yamashita, T., Tohyama, M., Shimada, S., Sato, K., and Sobue, K. (1997) J. Biol. Chem. 272, 15396-15404), and identified β-TM as an SMC-differentiation marker. Here, we characterized the transcriptional machinery of the β-TM gene in SMCs. Promoter and gel mobility shift analyses revealed an obligatory role for serum response factor and its interaction with the CArG box sequence in the SMC-specific transcription of the β-TM gene in differentiated SMCs. We further isolated a novel homologue of the Barx homeoprotein family, Barx1b, from chicken gizzard. Barx1b was exclusively localized to SMCs of the upper digestive organs and their attached arteries and to craniofacial structures. Serum response factor and Barx1b bound each other directly, coordinately transactivated the β-TM gene in differentiated SMCs and heterologous cells, and formed a ternary complex with a CArG probe. Taken together, these results suggest that SRF and Barx1b are coordinately involved in the SMC-specific transcription of the β-TM gene in the upper digestive organs and their attached arteries.

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