抄録
Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested ∼6000 yeast proteins for transcriptional activity using a yeast one-hybrid system and identified 451 transcriptional activators. We then determined their transcription activation strength using fusions to the Gal4 DNA-binding domain and a His3 reporter gene which contained a promoter with a Gal4-binding site. Among the 132 strongest activators 32 are known transcription factors while another 35 have no known function. Although zinc fingers, helix-loop-helix domains and several other domains are highly overrepresented among the activators, only few contain characterized ADs. We also found some striking correlations: the stronger the activation activity, the more acidic, glutamine-rich, proline-rich or asparagine-rich the activators were. About 29% of the activators have been found previously to specifically interact with the transcription machinery, while 10% are known to be components of transcription regulatory complexes. Based on their transcriptional activity, localization and interaction patterns, at least six previously uncharacterized proteins are suggested to be bona fide transcriptional regulators (namely YFL049W, YJR070C, YDR520C, YGL066W/Sgf73, YKR064W and YCR082W/Ahc2).
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 955-967 |
| ページ数 | 13 |
| ジャーナル | Nucleic acids research |
| 巻 | 34 |
| 発行部数 | 3 |
| DOI | |
| 出版物ステータス | 出版済み - 3 20 2006 |
| 外部発表 | Yes |
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All Science Journal Classification (ASJC) codes
- Genetics
これを引用
Transcriptional activators in yeast. / Titz, Björn; Thomas, Sindhu; Rajagopala, Seesandra V.; Chiba, Tomoko; Ito, Takashi; Uetz, Peter.
：: Nucleic acids research, 巻 34, 番号 3, 20.03.2006, p. 955-967.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Transcriptional activators in yeast
AU - Titz, Björn
AU - Thomas, Sindhu
AU - Rajagopala, Seesandra V.
AU - Chiba, Tomoko
AU - Ito, Takashi
AU - Uetz, Peter
PY - 2006/3/20
Y1 - 2006/3/20
N2 - Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested ∼6000 yeast proteins for transcriptional activity using a yeast one-hybrid system and identified 451 transcriptional activators. We then determined their transcription activation strength using fusions to the Gal4 DNA-binding domain and a His3 reporter gene which contained a promoter with a Gal4-binding site. Among the 132 strongest activators 32 are known transcription factors while another 35 have no known function. Although zinc fingers, helix-loop-helix domains and several other domains are highly overrepresented among the activators, only few contain characterized ADs. We also found some striking correlations: the stronger the activation activity, the more acidic, glutamine-rich, proline-rich or asparagine-rich the activators were. About 29% of the activators have been found previously to specifically interact with the transcription machinery, while 10% are known to be components of transcription regulatory complexes. Based on their transcriptional activity, localization and interaction patterns, at least six previously uncharacterized proteins are suggested to be bona fide transcriptional regulators (namely YFL049W, YJR070C, YDR520C, YGL066W/Sgf73, YKR064W and YCR082W/Ahc2).
AB - Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested ∼6000 yeast proteins for transcriptional activity using a yeast one-hybrid system and identified 451 transcriptional activators. We then determined their transcription activation strength using fusions to the Gal4 DNA-binding domain and a His3 reporter gene which contained a promoter with a Gal4-binding site. Among the 132 strongest activators 32 are known transcription factors while another 35 have no known function. Although zinc fingers, helix-loop-helix domains and several other domains are highly overrepresented among the activators, only few contain characterized ADs. We also found some striking correlations: the stronger the activation activity, the more acidic, glutamine-rich, proline-rich or asparagine-rich the activators were. About 29% of the activators have been found previously to specifically interact with the transcription machinery, while 10% are known to be components of transcription regulatory complexes. Based on their transcriptional activity, localization and interaction patterns, at least six previously uncharacterized proteins are suggested to be bona fide transcriptional regulators (namely YFL049W, YJR070C, YDR520C, YGL066W/Sgf73, YKR064W and YCR082W/Ahc2).
UR - http://www.scopus.com/inward/record.url?scp=33644849671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644849671&partnerID=8YFLogxK
U2 - 10.1093/nar/gkj493
DO - 10.1093/nar/gkj493
M3 - Article
C2 - 16464826
AN - SCOPUS:33644849671
VL - 34
SP - 955
EP - 967
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 3
ER -