Transcriptional activators in yeast

Björn Titz, Sindhu Thomas, Seesandra V. Rajagopala, Tomoko Chiba, Takashi Ito, Peter Uetz

研究成果: ジャーナルへの寄稿記事

69 引用 (Scopus)

抄録

Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested ∼6000 yeast proteins for transcriptional activity using a yeast one-hybrid system and identified 451 transcriptional activators. We then determined their transcription activation strength using fusions to the Gal4 DNA-binding domain and a His3 reporter gene which contained a promoter with a Gal4-binding site. Among the 132 strongest activators 32 are known transcription factors while another 35 have no known function. Although zinc fingers, helix-loop-helix domains and several other domains are highly overrepresented among the activators, only few contain characterized ADs. We also found some striking correlations: the stronger the activation activity, the more acidic, glutamine-rich, proline-rich or asparagine-rich the activators were. About 29% of the activators have been found previously to specifically interact with the transcription machinery, while 10% are known to be components of transcription regulatory complexes. Based on their transcriptional activity, localization and interaction patterns, at least six previously uncharacterized proteins are suggested to be bona fide transcriptional regulators (namely YFL049W, YJR070C, YDR520C, YGL066W/Sgf73, YKR064W and YCR082W/Ahc2).

元の言語英語
ページ(範囲)955-967
ページ数13
ジャーナルNucleic acids research
34
発行部数3
DOI
出版物ステータス出版済み - 3 20 2006
外部発表Yes

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Transcriptional Activation
Helix-Loop-Helix Motifs
Yeasts
Fungal Proteins
Asparagine
Zinc Fingers
Proteome
Glutamine
Reporter Genes
Proline
Transcription Factors
Binding Sites
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Titz, B., Thomas, S., Rajagopala, S. V., Chiba, T., Ito, T., & Uetz, P. (2006). Transcriptional activators in yeast. Nucleic acids research, 34(3), 955-967. https://doi.org/10.1093/nar/gkj493

Transcriptional activators in yeast. / Titz, Björn; Thomas, Sindhu; Rajagopala, Seesandra V.; Chiba, Tomoko; Ito, Takashi; Uetz, Peter.

:: Nucleic acids research, 巻 34, 番号 3, 20.03.2006, p. 955-967.

研究成果: ジャーナルへの寄稿記事

Titz, B, Thomas, S, Rajagopala, SV, Chiba, T, Ito, T & Uetz, P 2006, 'Transcriptional activators in yeast', Nucleic acids research, 巻. 34, 番号 3, pp. 955-967. https://doi.org/10.1093/nar/gkj493
Titz B, Thomas S, Rajagopala SV, Chiba T, Ito T, Uetz P. Transcriptional activators in yeast. Nucleic acids research. 2006 3 20;34(3):955-967. https://doi.org/10.1093/nar/gkj493
Titz, Björn ; Thomas, Sindhu ; Rajagopala, Seesandra V. ; Chiba, Tomoko ; Ito, Takashi ; Uetz, Peter. / Transcriptional activators in yeast. :: Nucleic acids research. 2006 ; 巻 34, 番号 3. pp. 955-967.
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