Transcriptional regulation of laccase and cellulase genes in the mycelium of Agaricus bisporus during fruit body development on a solid substrate

Shoji Ohga, Melvyn Smith, Christopher F. Thurston, David A. Wood

研究成果: ジャーナルへの寄稿記事

37 引用 (Scopus)

抄録

The cultivated mushroom Agaricus bisporus was grown and fruited in wheat straw compost. RNA was extracted from samples of mycelium growing in compost during colonization and fruit body development. Laccase gene (combined lcc1 and lcc2) expression and cel3 gene (cellobiohydrolase) expression were determined by Northern blot analysis and by competitive RT-PCR. The level of laccase transcripts was highest in colonized compost (prior to the onset of fruiting), declined during the period of fruit body enlargement and increased again after harvesting and during the second flush of fruit body production. No transcripts of the cel3 gene were detectable in colonized or pre-fruiting cultures, but the level became detectable and rose to a maximum at the veil-break stage of fruiting, declined after harvesting and rose again during the second flush. Gene expression for laccase and cellobiohydrolase correlated with the known changes of laccase and cellulase enzyme activities during the fruiting life cycle.

元の言語英語
ページ(範囲)1557-1560
ページ数4
ジャーナルMycological Research
103
発行部数12
DOI
出版物ステータス出版済み - 12 1999

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Agaricus
Laccase
Agaricus bisporus
Cellulase
laccase
Mycelium
fruiting
fruiting bodies
endo-1,4-beta-glucanase
mycelium
Fruit
fruit
Cellulose 1,4-beta-Cellobiosidase
cellulose 1,4-beta-cellobiosidase
compost
composts
substrate
Soil
gene
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

これを引用

Transcriptional regulation of laccase and cellulase genes in the mycelium of Agaricus bisporus during fruit body development on a solid substrate. / Ohga, Shoji; Smith, Melvyn; Thurston, Christopher F.; Wood, David A.

:: Mycological Research, 巻 103, 番号 12, 12.1999, p. 1557-1560.

研究成果: ジャーナルへの寄稿記事

Ohga, Shoji ; Smith, Melvyn ; Thurston, Christopher F. ; Wood, David A. / Transcriptional regulation of laccase and cellulase genes in the mycelium of Agaricus bisporus during fruit body development on a solid substrate. :: Mycological Research. 1999 ; 巻 103, 番号 12. pp. 1557-1560.
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