Transcriptional up-regulation of p27Kip1 during contact-induced growth arrest in vascular endothelial cells

Mayumi Hirano, Katsuya Hirano, Junji Nishimura, Hideo Kanaide

研究成果: ジャーナルへの寄稿記事

32 引用 (Scopus)

抄録

By plating porcine aortic endothelial cells at two different densities and thereby inducing two different time courses of contact-induced growth arrest, the temporal correlation between p27Kip1 expression and cell cycle progression was investigated. When the quiescent cells were replated, they synchronously entered S phase with a peak at 20 h in both cases, while the cells plated at 25 and 80% of confluent densities exited the cell cycle by 96 and 48 h, respectively. Nuclear p27Kip1 disappeared when the cells reentered the cell cycle and then recovered when the cells exited the cell cycle. The change in p27Kip1 was associated with a concomitant change in Kip1 mRNA. The p27Kip1 degradation activity did not increase in the cells reentering the cell cycle, nor did it decrease in the cells exiting the cell cycle. The Kip1 mRNA stability decreased in the growing cells and increased in the cells exiting the cell cycle and at confluence. A nuclear run-on assay revealed a close correlation between the Kip1 transcriptional activity and the level of Kip1 mRNA. We conclude that the cell-cell contact up-regulated the Kip1 gene transcription and increased the Kip1 mRNA stability, which was related to the recovery of p27Kip1 protein during contact-induced growth arrest in endothelial cells.

元の言語英語
ページ(範囲)356-367
ページ数12
ジャーナルExperimental Cell Research
271
発行部数2
DOI
出版物ステータス出版済み - 12 10 2001

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Up-Regulation
Endothelial Cells
Cell Cycle
Growth
RNA Stability
Cyclin-Dependent Kinase Inhibitor p27
Messenger RNA
S Phase
Swine
Genes

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

Transcriptional up-regulation of p27Kip1 during contact-induced growth arrest in vascular endothelial cells. / Hirano, Mayumi; Hirano, Katsuya; Nishimura, Junji; Kanaide, Hideo.

:: Experimental Cell Research, 巻 271, 番号 2, 10.12.2001, p. 356-367.

研究成果: ジャーナルへの寄稿記事

Hirano, Mayumi ; Hirano, Katsuya ; Nishimura, Junji ; Kanaide, Hideo. / Transcriptional up-regulation of p27Kip1 during contact-induced growth arrest in vascular endothelial cells. :: Experimental Cell Research. 2001 ; 巻 271, 番号 2. pp. 356-367.
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