Transcriptome complexity in a genome-reduced bacterium

Marc Güell, Vera Van Noort, Eva Yus, Wei Hua Chen, Justine Leigh-Bell, Konstantinos Michalodimitrakis, Takuji Yamada, Manimozhiyan Arumugam, Tobias Doerks, Sebastian Kühner, Michaela Rode, Mikita Suyama, Sabine Schmidt, Anne Claude Gavin, Peer Bork, Luis Serrano

研究成果: Contribution to journalArticle

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To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms. Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

元の言語英語
ページ(範囲)1268-1271
ページ数4
ジャーナルScience
326
発行部数5957
DOI
出版物ステータス出版済み - 11 27 2009
外部発表Yes

All Science Journal Classification (ASJC) codes

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    Güell, M., Van Noort, V., Yus, E., Chen, W. H., Leigh-Bell, J., Michalodimitrakis, K., Yamada, T., Arumugam, M., Doerks, T., Kühner, S., Rode, M., Suyama, M., Schmidt, S., Gavin, A. C., Bork, P., & Serrano, L. (2009). Transcriptome complexity in a genome-reduced bacterium. Science, 326(5957), 1268-1271. https://doi.org/10.1126/science.1176951