Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast

Tohru Ichimura, Hiroyuki Kubota, Takeshi Goma, Noboru Mizushima, Yoshinori Ohsumi, Maki Iwago, Kazue Kakiuchi, Hossain Uddin Shekhar, Takashi Shinkawa, Masato Taoka, Takashi Ito, Toshiaki Isobe

研究成果: ジャーナルへの寄稿記事

32 引用 (Scopus)

抄録

BMH1 and BMH2 encode Saccharomyces cerevisiae 14-3-3 homologues whose exact functions have remained unclear. The present work compares the transcriptomic and proteomic profiles of the wild type and a BMH1/2-deficient S. cerevisiae mutant (bmhΔ) using DNA microarrays and two-dimensional polyacrylamide gel electrophoresis. It is reported here that, although the global patterns of gene and protein expression are very similar between the two types of yeast cells, a subset of genes and proteins (a total of 220 genes) is significantly induced or reduced in the absence of Bmh1/2p. These genes include approximately 60 elements that could be linked to the reported phenotypes of the bmhΔ mutant (e.g., accumulation of glycogen and hypersensitivity to environmental stress) and/or could be the potential downstream targets of interacting partners of Bmh1/2p such as Msn2p and Rtg3p. Importantly, >30% of the identified genes (71 genes) were found to be associated with carbon (C) and nitrogen (N) metabolism and transport, thereby suggesting that Bmh1/2p may play a major role in the regulation of C/N-responsive cellular processes. This study presents the first comprehensive overview of the genes and proteins that are affected by the depletion of Bmh1/2p and extends the scope of knowledge of the regulatory roles of Bmh1/2p in S. cerevisiae.

元の言語英語
ページ(範囲)6149-6158
ページ数10
ジャーナルBiochemistry
43
発行部数20
DOI
出版物ステータス出版済み - 5 25 2004
外部発表Yes

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Proteomics
Yeast
Genes
Yeasts
Saccharomyces cerevisiae
Environmental Illness
Proteins
Electrophoresis, Gel, Two-Dimensional
Microarrays
Oligonucleotide Array Sequence Analysis
Electrophoresis
Glycogen
Metabolism
Nitrogen
Carbon
Cells
Phenotype
Gene Expression
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

Ichimura, T., Kubota, H., Goma, T., Mizushima, N., Ohsumi, Y., Iwago, M., ... Isobe, T. (2004). Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast. Biochemistry, 43(20), 6149-6158. https://doi.org/10.1021/bi035421i

Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast. / Ichimura, Tohru; Kubota, Hiroyuki; Goma, Takeshi; Mizushima, Noboru; Ohsumi, Yoshinori; Iwago, Maki; Kakiuchi, Kazue; Shekhar, Hossain Uddin; Shinkawa, Takashi; Taoka, Masato; Ito, Takashi; Isobe, Toshiaki.

:: Biochemistry, 巻 43, 番号 20, 25.05.2004, p. 6149-6158.

研究成果: ジャーナルへの寄稿記事

Ichimura, T, Kubota, H, Goma, T, Mizushima, N, Ohsumi, Y, Iwago, M, Kakiuchi, K, Shekhar, HU, Shinkawa, T, Taoka, M, Ito, T & Isobe, T 2004, 'Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast', Biochemistry, 巻. 43, 番号 20, pp. 6149-6158. https://doi.org/10.1021/bi035421i
Ichimura T, Kubota H, Goma T, Mizushima N, Ohsumi Y, Iwago M その他. Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast. Biochemistry. 2004 5 25;43(20):6149-6158. https://doi.org/10.1021/bi035421i
Ichimura, Tohru ; Kubota, Hiroyuki ; Goma, Takeshi ; Mizushima, Noboru ; Ohsumi, Yoshinori ; Iwago, Maki ; Kakiuchi, Kazue ; Shekhar, Hossain Uddin ; Shinkawa, Takashi ; Taoka, Masato ; Ito, Takashi ; Isobe, Toshiaki. / Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast. :: Biochemistry. 2004 ; 巻 43, 番号 20. pp. 6149-6158.
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abstract = "BMH1 and BMH2 encode Saccharomyces cerevisiae 14-3-3 homologues whose exact functions have remained unclear. The present work compares the transcriptomic and proteomic profiles of the wild type and a BMH1/2-deficient S. cerevisiae mutant (bmhΔ) using DNA microarrays and two-dimensional polyacrylamide gel electrophoresis. It is reported here that, although the global patterns of gene and protein expression are very similar between the two types of yeast cells, a subset of genes and proteins (a total of 220 genes) is significantly induced or reduced in the absence of Bmh1/2p. These genes include approximately 60 elements that could be linked to the reported phenotypes of the bmhΔ mutant (e.g., accumulation of glycogen and hypersensitivity to environmental stress) and/or could be the potential downstream targets of interacting partners of Bmh1/2p such as Msn2p and Rtg3p. Importantly, >30{\%} of the identified genes (71 genes) were found to be associated with carbon (C) and nitrogen (N) metabolism and transport, thereby suggesting that Bmh1/2p may play a major role in the regulation of C/N-responsive cellular processes. This study presents the first comprehensive overview of the genes and proteins that are affected by the depletion of Bmh1/2p and extends the scope of knowledge of the regulatory roles of Bmh1/2p in S. cerevisiae.",
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AU - Shekhar, Hossain Uddin

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