Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity

Jie Gu, Jing Woei Li, William Ka Fai Tse, Ting Fung Chan, Keng Po Lai, Chris Kong Chu Wong

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

Physiological studies of a unique endocrine gland in fish, named corpuscles of Stannius (CS), described a Ca2 + -regulatory function for this gland mediated by stanniocalcin-1, a hypocalcemic polypeptide hormone. However, to date, the endocrine functions of the glands have not been completely elucidated.We hypothesized that other unidentified active principles in the glands are involved in the regulation of plasma ion (Na +, Ca2 +) and/or blood pressure. In this study, transcriptome sequencing of CS glands was performed using Japanese eels (Anguilla japonica) adapted to freshwater (FW) or seawater (SW) to reveal the presence and differential expression of genes encoding proteins related to the ion-osmoregulatory and pressor functions. We acquired a total of 14.1 Mb and 12.1 Mb quality-trimmed reads from the CS glands collected from FW and SW adapted eels, respectively. The de novo assembly resulted in 9254 annotated genes. Among them, 475 genes were differentially expressed with 357 up- and 118 down-regulated in the SW group. Gene ontology analysis further demonstrated the presence of natriuresis and pressor related genes. In summary, ours is the first study using high-throughput sequencing to identify gene targets that could explain the physiological importance of the CS glands.

元の言語英語
記事番号9836
ジャーナルScientific reports
5
DOI
出版物ステータス出版済み - 4 23 2015

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Anguilla
Eels
Salinity
Seawater
Endocrine Glands
Water
Fresh Water
Genes
Ions
Natriuresis
Gene Ontology
Peptide Hormones
Transcriptome
Fishes
Blood Pressure
Proteins

All Science Journal Classification (ASJC) codes

  • General

これを引用

Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity. / Gu, Jie; Li, Jing Woei; Tse, William Ka Fai; Chan, Ting Fung; Lai, Keng Po; Wong, Chris Kong Chu.

:: Scientific reports, 巻 5, 9836, 23.04.2015.

研究成果: ジャーナルへの寄稿記事

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abstract = "Physiological studies of a unique endocrine gland in fish, named corpuscles of Stannius (CS), described a Ca2 + -regulatory function for this gland mediated by stanniocalcin-1, a hypocalcemic polypeptide hormone. However, to date, the endocrine functions of the glands have not been completely elucidated.We hypothesized that other unidentified active principles in the glands are involved in the regulation of plasma ion (Na +, Ca2 +) and/or blood pressure. In this study, transcriptome sequencing of CS glands was performed using Japanese eels (Anguilla japonica) adapted to freshwater (FW) or seawater (SW) to reveal the presence and differential expression of genes encoding proteins related to the ion-osmoregulatory and pressor functions. We acquired a total of 14.1 Mb and 12.1 Mb quality-trimmed reads from the CS glands collected from FW and SW adapted eels, respectively. The de novo assembly resulted in 9254 annotated genes. Among them, 475 genes were differentially expressed with 357 up- and 118 down-regulated in the SW group. Gene ontology analysis further demonstrated the presence of natriuresis and pressor related genes. In summary, ours is the first study using high-throughput sequencing to identify gene targets that could explain the physiological importance of the CS glands.",
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