Transforming growth factor β1 promotes migration of human periodontal ligament cells through heat shock protein 27 phosphorylation

Seong Min Kwon, Soo A. Kim, Shinsuke Fujii, Hidefumi Maeda, Sang Gun Ahn, Jung Hoon Yoon

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

Transforming growth factor β1 (TGF- β1) regulates cellular functions including cell growth, differentiation, and migration. However, signal transduction pathways of TGF- β1 are mostly undefined in human periodontal ligament (hPDL) cells. In this study, we investigated TGF β1-induced migration focusing on heat shock protein 27 (Hsp27) activation. The cellular response to TGF- β1 by hPDL cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Western blot analysis. Cell migration was determined by counting migrated cells using a Chemotaxis Cell Migration Assay kit. TGF- β1 induced cell migration and increased the phosphorylation of Hsp27 and p38 mitogen-activated protein kinase (MAPK) in hPDL cells. Inhibiting p38 MAPK with SB203580 blocked TGF- β1-induced Hsp27 activation and cell migration. These findings suggest that TGF- β1-induced Hsp27 may promote the migration of hPDL cells via p38 MAPK mediated signaling. Hsp27 may be a potential target for the periodontal regeneration process related to cell migration.

本文言語英語
ページ(範囲)486-489
ページ数4
ジャーナルBiological and Pharmaceutical Bulletin
34
4
DOI
出版ステータス出版済み - 4 2011

All Science Journal Classification (ASJC) codes

  • 薬理学
  • 薬科学

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