Transgenesis and imaging in birds, and available transgenic reporter lines

Yuki Sato, Rusty Lansford

研究成果: ジャーナルへの寄稿評論記事

14 引用 (Scopus)

抄録

Avian embryos are important model organism to study higher vertebrate development. Easy accessibility to developing avian embryos enables a variety of experimental applications to understand specific functions of molecules, tissue-tissue interactions, and cell lineages. The whole-mount ex ovo culture technique for avian embryos permits time-lapse imaging analysis for a better understanding of cell behaviors underlying tissue morphogenesis in physiological conditions. To study mechanisms of blood vessel formation and remodeling in developing embryos by using a time-lapse imaging approach, a transgenic quail model, Tg(tie1:H2B-eYFP), was generated. From a cell behavior perspective, Tg(tie1:H2B-eYFP) quail embryos are a suitable model to shed light on how the structure and pattern of blood vessels are established in higher vertebrates. In this manuscript, we give an overview on the biological and technological background of the transgenic quail model and describe procedures for the ex ovo culture of quail embryos and time-lapse imaging analysis. Development, Growth & Differentiation

元の言語英語
ページ(範囲)406-421
ページ数16
ジャーナルDevelopment Growth and Differentiation
55
発行部数4
DOI
出版物ステータス出版済み - 5 1 2013

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Gene Transfer Techniques
Quail
Birds
Time-Lapse Imaging
Embryonic Structures
Embryo Culture Techniques
Blood Vessels
Vertebrates
Cell Lineage
Morphogenesis
Growth and Development

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

これを引用

Transgenesis and imaging in birds, and available transgenic reporter lines. / Sato, Yuki; Lansford, Rusty.

:: Development Growth and Differentiation, 巻 55, 番号 4, 01.05.2013, p. 406-421.

研究成果: ジャーナルへの寄稿評論記事

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