Translation initiation: A regulatory role for poly(A) tracts in front of the AUG codon in saccharomyces cerevisiae

Xuhua Xia, Vivian Mackay, Xiaoquan Yao, Jianhua Wu, Fumihito Miura, Takashi Ito, David R. Morris

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

The 59-UTR serves as the loading dock for ribosomes during translation initiation and is the key site for translation regulation. Many genes in the yeast Saccharomyces cerevisiae contain poly(A) tracts in their 59-UTRs. We studied these pre-AUG poly(A) tracts in a set of 3274 recently identified 59-UTRs in the yeast to characterize their effect on in vivo protein abundance, ribosomal density, and protein synthesis rate in the yeast. The protein abundance and the protein synthesis rate increase with the length of the poly(A), but exhibit a dramatic decrease when the poly(A) length is ≥12. The ribosomal density also reaches the lowest level when the poly(A) length is ≥12. This supports the hypothesis that a pre-AUG poly(A) tract can bind to translation initiation factors to enhance translation initiation, but a long (≥12) pre-AUG poly(A) tract will bind to Pab1p, whose binding size is 12 consecutive A residues in yeast, resulting in repression of translation. The hypothesis explains why a long pre-AUG poly(A) leads to more efficient translation initiation than a short one when PABP is absent, and why pre-AUG poly(A) is short in the early genes but long in the late genes of vaccinia virus.

元の言語英語
ページ(範囲)469-478
ページ数10
ジャーナルGenetics
189
発行部数2
DOI
出版物ステータス出版済み - 10 1 2011
外部発表Yes

Fingerprint

Poly A
Codon
Saccharomyces cerevisiae
Untranslated Regions
Yeasts
Genes
Peptide Initiation Factors
Proteins
Vaccinia virus
Protein Biosynthesis
Ribosomes

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Translation initiation : A regulatory role for poly(A) tracts in front of the AUG codon in saccharomyces cerevisiae. / Xia, Xuhua; Mackay, Vivian; Yao, Xiaoquan; Wu, Jianhua; Miura, Fumihito; Ito, Takashi; Morris, David R.

:: Genetics, 巻 189, 番号 2, 01.10.2011, p. 469-478.

研究成果: ジャーナルへの寄稿記事

Xia, Xuhua ; Mackay, Vivian ; Yao, Xiaoquan ; Wu, Jianhua ; Miura, Fumihito ; Ito, Takashi ; Morris, David R. / Translation initiation : A regulatory role for poly(A) tracts in front of the AUG codon in saccharomyces cerevisiae. :: Genetics. 2011 ; 巻 189, 番号 2. pp. 469-478.
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