TY - JOUR
T1 - Transport of Organic Acids through a Supported Liquid Membrane Driven by Lipase-Catalyzed Reactions
AU - Miyako, Eijiro
AU - Maruyama, Tatsuo
AU - Kamiya, Noriho
AU - Goto, Masahiro
N1 - Funding Information:
This work was partly supported by a Grand-in-Aid for Scientific Research (no. 13 129205) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We are grateful to Amano Enzyme Inc. for generously providing the enzymes.
PY - 2003
Y1 - 2003
N2 - We have developed a lipase-facilitated supported liquid membrane. Lipase-catalyzed reactions were coupled with a supported liquid membrane (SLM) to transport organic acids through the SLM. We succeeded in the rational transport of organic acids through the SLM using lipase-catalyzed reactions and observed that there were differences in the transport behavior of various organic acids due to the substrate specificity of lipase. Subsequently, various parameters, such as the alcohol concentration in the feed phase, the pH in each aqueous phase, an organic solvent in the SLM, and the kind of lipase, were investigated. We found that the optimum conditions were 65 vol% alcohol concentration, pH 6.3 in each aqueous phase, isooctane as the liquid membrane phase and Candida rugosa lipase as the esterification biocatalyst.
AB - We have developed a lipase-facilitated supported liquid membrane. Lipase-catalyzed reactions were coupled with a supported liquid membrane (SLM) to transport organic acids through the SLM. We succeeded in the rational transport of organic acids through the SLM using lipase-catalyzed reactions and observed that there were differences in the transport behavior of various organic acids due to the substrate specificity of lipase. Subsequently, various parameters, such as the alcohol concentration in the feed phase, the pH in each aqueous phase, an organic solvent in the SLM, and the kind of lipase, were investigated. We found that the optimum conditions were 65 vol% alcohol concentration, pH 6.3 in each aqueous phase, isooctane as the liquid membrane phase and Candida rugosa lipase as the esterification biocatalyst.
UR - http://www.scopus.com/inward/record.url?scp=0345059756&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345059756&partnerID=8YFLogxK
U2 - 10.1263/jbb.96.370
DO - 10.1263/jbb.96.370
M3 - Article
C2 - 16233539
AN - SCOPUS:0345059756
SN - 1389-1723
VL - 96
SP - 370
EP - 374
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 4
ER -