The transportation of reactive oxygen species (ROSs) in a tissue phantom after plasma irradiation was studied using a two-layered target consisting of a KI-starch gel reagent and an agarose tissue phantom. The two-layered target can visualize the two-dimensional concentration distribution of ROSs after passing through the tissue phantom. ROSs were accumulated in the tissue phantom by the plasma irradiation, and they continued to be transported in the depth direction with the standing time after the plasma irradiation. The amount of ROS after passing through the tissue phantom increased in proportion to both plasma irradiation time and standing time. In this case, the ROS distribution patterns did not depend on these times. The ROS transportation speed after plasma irradiation was 0.05 mm/min in the tissue phantom. The ROS penetration rate depended on the standing time, not on the plasma irradiation time, and it was less than 1%.
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