Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells

Junya Kawasaki, Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Masatoshi Fujishima, Hideo Kanaide

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

To investigate the effects of troglitazone on the capacitative Ca2+ entry, we monitored changes in cytosolic Ca2+ concentrations ([Ca2+](i)) induced by thapsigargin in fura-2-loaded porcine endothelial cells in situ and in primary culture. In aortic valve endothelial cells in situ, thapsigargin induced sustained elevation of [Ca2+](i). Both troglitazone and SKF 96365 inhibited the steady state increase in [Ca2+](i) in a concentration-dependent manner. At 30 μM, troglitazone and SKF 96365 inhibited the [Ca2+](i) elevation to 19.4 ± 3.6% and 43.9 ± 4.5%, respectively. In aortic endothelial cells in primary culture, both troglitazone (10 μM) and SKF 96365 (100 μM) completely inhibited the thapsigargin-induced [Ca2+](i) increase. The EC50 value of troglitazone (1.4 ± 0.1 μM) was lower than that of SKF 96365 (10.0 ± 3.3 μM). We suggest that troglitazone would be a useful tool to investigate the capacitative Ca2+ entry.

元の言語英語
ページ(範囲)111-120
ページ数10
ジャーナルEuropean Journal of Pharmacology
373
発行部数1
DOI
出版物ステータス出版済み - 5 28 1999
外部発表Yes

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troglitazone
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Endothelial Cells
Thapsigargin
Fura-2
Aortic Valve
Swine

All Science Journal Classification (ASJC) codes

  • Pharmacology

これを引用

Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells. / Kawasaki, Junya; Hirano, Katsuya; Hirano, Mayumi; Nishimura, Junji; Fujishima, Masatoshi; Kanaide, Hideo.

:: European Journal of Pharmacology, 巻 373, 番号 1, 28.05.1999, p. 111-120.

研究成果: ジャーナルへの寄稿記事

Kawasaki, J, Hirano, K, Hirano, M, Nishimura, J, Fujishima, M & Kanaide, H 1999, 'Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells', European Journal of Pharmacology, 巻. 373, 番号 1, pp. 111-120. https://doi.org/10.1016/S0014-2999(99)00257-5
Kawasaki, Junya ; Hirano, Katsuya ; Hirano, Mayumi ; Nishimura, Junji ; Fujishima, Masatoshi ; Kanaide, Hideo. / Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells. :: European Journal of Pharmacology. 1999 ; 巻 373, 番号 1. pp. 111-120.
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