Tsix-deficient X chromosome does not undergo inactivation in the embryonic lineage in males: Implications for Tsix-independent silencing of Xist

T. Ohhata, Y. Hoki, Hiroyuki Sasaki, T. Sado

研究成果: Contribution to journalArticle査読

27 被引用数 (Scopus)

抄録

Differential induction of the X-linked non-coding Xist gene is a key event in the process of X inactivation occurring in female mammalian embryos. Xist is negatively regulated in cis by its antisense gene Tsix through modification of the chromatin structure. The maternal Xist allele, which is normally silent in the extraembryonic lineages, is ectopically activated when Tsix is disrupted on the same chromosome, and subsequently the maternal X chromosome undergoes inactivation in the extraembryonic lineages even in males. However, it is still unknown whether the single Tsix-deficient X chromosome (X ΔTsix) in males is also inactivated in the embryonic lineage. Here, we show that both male and female embryos carrying a maternally derived X ΔTsix could survive if the extraembryonic tissues were complemented by wild-type tetraploid cells. In addition, Xist on the X ΔTsix was properly silenced and methylated at CpG sites in adult male somatic cells. These results indicate that the embryonic lethality caused by the maternal X ΔTsix is solely attributable to the defects in the extraembryonic lineages. X ΔTsix does not seem to undergo inactivation in the embryonic lineage in males, suggesting the presence of a Tsix-independent silencing mechanism for Xist in the embryonic lineage.

本文言語英語
ページ(範囲)345-349
ページ数5
ジャーナルCytogenetic and Genome Research
113
1-4
DOI
出版ステータス出版済み - 3 2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

フィンガープリント 「Tsix-deficient X chromosome does not undergo inactivation in the embryonic lineage in males: Implications for Tsix-independent silencing of Xist」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル