Tumor-derived interleukin-1 promotes lymphangiogenesis and lymph node metastasis through M2-type macrophages

Kosuke Watari, Tomohiro Shibata, Akihiko Kawahara, Ken Ichi Sata, Hiroshi Nabeshima, Ai Shinoda, Hideyuki Abe, Koichi Azuma, Yuichi Murakami, Hiroto Izumi, Takashi Takahashi, Masayoshi Kage, Michihiko Kuwano, Mayumi Ono

研究成果: Contribution to journalArticle査読

42 被引用数 (Scopus)

抄録

Tumors formed by a highly metastatic human lung cancer cell line are characterized by activated signaling via vascular endothelial growth factor (VEGF)-C through its receptor (VEGFR-3) and aggressive lymph node metastasis. In this study, we examined how these highly metastatic cancers acquired aggressive lymph node metastasis. Compared with their lower metastatic counterparts, the highly metastatic tumors formed by this cell line expressed higher amounts of interleukin (IL)-1α, with similarly augmented expression of IL-1α and IL-1β by tumor stromal cells and of VEGF-A and VEGF-C by tumorassociated macrophages. These tumor-associated macrophages were mainly of the M2 type. Administration of a macrophage-targeting drug suppressed the production of these potent angiogenic and lymphangiogenic factors, resulting in decreased tumor growth, angiogenesis, lymphangiogenesis, and lymph node metastasis. In Matrigel plug assays, the highly metastatic cells formed tumors that were extensively infiltrated by M2-type macrophages and exhibited enhanced angiogenesis and lymphangiogenesis. All of these responses were suppressed by the IL-1 receptor (IL-1R) antagonist anakinra. Thus, the IL-1α-driven inflammatory activation of angiogenesis and lymphangiogenesis seems to provide a highly metastatic tumor microenvironment favorable for lymph node metastasis through cross-talk with macrophages. Accordingly, the IL-1R/M2-type macrophage axis may be a good therapeutic target for patients with this form of lung cancer.

本文言語英語
論文番号e99568
ジャーナルPloS one
9
6
DOI
出版ステータス出版済み - 6 12 2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

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