Turn-on fluorescence sensing of nucleoside polyphosphates using a xanthene-based Zn(II) complex chemosensor

Akio Ojida, Ippei Takashima, Takahiro Kohira, Hiroshi Nonaka, Itaru Hamachi

研究成果: ジャーナルへの寄稿記事

230 引用 (Scopus)

抄録

Fluorescence sensing with small molecular chemosensors is a versatile technique for elucidation of function of various biological substances. We now report a new fluorescent chemosensor for nucleoside polyphosphates such as ATP using metal-anion coordination chemistry. The chemosensor 1-2Zn(II) is comprised of the two sites of 2,27prime;-dipicolylamine (Dpa)-Zn(II) as the binding motifs and xanthene as a fluorescent sensing unit for nucleoside polyphosphates. The chemosensor 1-2Zn(II) selectively senses nucleoside polyphosphates with a large fluorescence enhancement (F/Fo > 15) and strong binding affinity (Kapp ≈ 1 × 106 M-1), whereas no detectable fluorescence change was induced by monophosphate species and various other anions. The 'turn-on,' fluorescence of 1-2Zn(II) is based on a new mechanism, which involves the binding-induced recovery of the conjugated form of the xanthene ring from its nonfluorescent deconjugated state which was formed by an unprecedented nucleophilic attack of zinc-bound water. The selective and highly sensitive ability of 1-2Zn(II) to detect nucleoside polyphosphates enables its bioanalytical applications in fluorescence visualization of ATP particulate stores in living cells, demonstrating the potential utility of 1-2Zn(II).

元の言語英語
ページ(範囲)12095-12101
ページ数7
ジャーナルJournal of the American Chemical Society
130
発行部数36
DOI
出版物ステータス出版済み - 9 10 2008

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Xanthenes
Polyphosphates
Nucleosides
Fluorescence
Adenosinetriphosphate
Anions
Negative ions
Adenosine Triphosphate
Zinc
Visualization
Metals
Cells
Recovery
Water

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

これを引用

Turn-on fluorescence sensing of nucleoside polyphosphates using a xanthene-based Zn(II) complex chemosensor. / Ojida, Akio; Takashima, Ippei; Kohira, Takahiro; Nonaka, Hiroshi; Hamachi, Itaru.

:: Journal of the American Chemical Society, 巻 130, 番号 36, 10.09.2008, p. 12095-12101.

研究成果: ジャーナルへの寄稿記事

Ojida, Akio ; Takashima, Ippei ; Kohira, Takahiro ; Nonaka, Hiroshi ; Hamachi, Itaru. / Turn-on fluorescence sensing of nucleoside polyphosphates using a xanthene-based Zn(II) complex chemosensor. :: Journal of the American Chemical Society. 2008 ; 巻 130, 番号 36. pp. 12095-12101.
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