Twigged streptavidin polymer as a scaffold for protein assembly

Takuya Matsumoto, Yuki Isogawa, Kosuke Minamihata, Tsutomu Tanaka, Akihiko Kondo

研究成果: ジャーナルへの寄稿学術誌査読

9 被引用数 (Scopus)

抄録

Protein assemblies are an emerging tool that is finding many biological and bioengineering applications. We here propose a method for the site-specific assembly of proteins on a twigged streptavidin (SA) polymer using streptavidin as a functional scaffold. SA was genetically appended with a G tag (sortase A recognition sequence) and a Y tag (HRP recognition sequence) on its N- and C-termini, respectively, to provide G-SA-Y. G-SA-Y was polymerized using HPR-mediated tyrosine coupling, then fluorescent proteins were immobilized on the polymer by biotin-SA affinity and sortase A-mediated ligation. Fluorescence measurements showed that the proteins were immobilized in close proximity to each other. Hydrolyzing enzymes were also functionally assembled on the G-SA-Y polymer. The site-specific assembly of proteins on twigged SA polymer may find new applications in various biological and bioengineering fields.

本文言語英語
ページ(範囲)61-66
ページ数6
ジャーナルJournal of Biotechnology
225
DOI
出版ステータス出版済み - 5月 10 2016
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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